Effects of Shenfu Yixin Decoction on the Utilization of Fatty Acid in Primary Hypoxic Cardiomyocytes
- VernacularTitle:参附益心方对缺氧原代心肌细胞脂肪酸利用的影响
- Author:
Lijie QIAO
1
,
2
;
Bin LI
1
;
Xinlu WANG
1
;
Shiyang XIE
3
;
Yuan GAO
3
;
Mingjun ZHU
1
;
Yongxia WANG
1
Author Information
1. Heart Center，the First Affiliated Hospital of Henan University of TCM，Zhengzhou 450099，China
2. First College of Clinical Medicine，Henan University of TCM，Zhengzhou 450099，China
3. Central Laboratory，the First Affiliated Hosp ital of Henan University of TCM，Zhengzhou 450099，China
- Publication Type:Journal Article
- Keywords:
Hypoxia;
Primary cardiomyocytes;
Fatty acid;
Metabolism;
Shenfu yixin decoction
- From:
China Pharmacy
2020;31(2):149-153
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To investigate the effects of Shenfu yixin decoction on the utilization of fatty acid in primary hypoxic cardiomyocytes and its potential mechanism. METHODS ：The apical tissue of neonatal SD rats with 1-3 days old were collected ， and the primary cardiomyocytes were isolated ，cultured and identified. The cardiomyocytes were randomly divided into normal group，model group ，coenzyme Q 10 group（positive control ，1×10－4 mol/L），Shenfu yixin decoction low-dose and high-dose groups（0.25，0.5 mg/mL）. Except for normal group ，cells in other groups were cultured under 5％O2，5％CO2 and 90％N2 for 6 hours to induce hypoxic injury model. After 6 hours of hypoxia ，the content of ATP was detected by luciferase luminescence assay. Western blotting assay was adopted to detect the expression of FAT/CD 36，PPARα and PPARβ/δ. RESULTS：Compared with normal group ，the content of ATP and relative expression of FAT/CD 36 protein were decreased significantly in model group （P＜ 0.05）. Compared with model group ，the content of ATP was increased significantly in coenzyme Q 10 group and Shenfu yixin decoction high-dose group ，while the relative expression of FAT/CD 36 and PPARα protein in coenzyme Q10 group，the relative expression of FAT/CD 36 protein in Shenfu yixin decoction high-dose group as well as the relative expression of PPARα and PPARβ/δ protein in Shenfu yixin decoction groups were decreased significantly （P＜0.05）. CONCLUSIONS ：Shenfu yixin decoction can inhibit the utilization of fatty acid of primary hypoxic cardiomyocytes and improve their energy metabolism by inhibiting the expression of FAT/CD 36，PPARα and PPARβ/δ protein.
