Study on Extraction Technology Optimization and Structural Characterization and of Fritillaria pallidiflora Poly- saccharide
- VernacularTitle:伊贝母多糖的提取工艺优化及结构表征
- Author:
Abuduwaili AYITUXUN
1
,
2
;
Rouzi PAERHATI
3
;
Yanhua GAO
1
;
Mutailifu PAIHEERDING
1
,
2
;
Nuerxiati REHEBATI
1
,
2
;
Huixia NING
1
;
Yili ABULIMITI
1
Author Information
1. Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization，Xinjiang Technical Institute of Physics and Chemistry，Chinese Academy of Sciences，Urumqi 830011，China
2. University of the Chinese Academy of Sciences，Beijing 100049，China
3. College of Food Science and Pharmacy，Xinjiang Agricultural Universit y，Urumqi 830052，China
- Publication Type:Journal Article
- Keywords:
Fritillaria pallidiflora polysaccharide;
Box-Behnken design;
Response surface methodology;
Structural
- From:
China Pharmacy
2020;31(3):276-281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To optimize the extractio n technology of Fritillaria pallidiflora polysaccharides（FPSP），and to study its structure. METHODS ：Using the yield of FPSP as response value ，Box-Behnken design-response surface methodology was adopted to optimize solid-liquid ratio ，extraction temperature and extraction time of FPSP extraction technology. Structural properties of FPSP was characterized by UV spectrum ，FTIR，GC-MS，Congo red staining ，SEM，XRD and thermogravimetric analysis. RESULTS：The optimal technology parameters of FPSP were solid-liquid ratio of 1∶28（g/mL），extraction temperature of 94 ℃， extraction time of 2.5 h；the yield of FPSP was 16.25％（n＝3），the error of which to theoretical yield （16.58％）was 0.33％. FPSP contained xylose ，glucose and galactose with a molar ratio of 1∶58.02∶0.73，and trace amount of mannose ；there was a weak absorption peak near the wavelength of 280 nm；belonged to α-configuration pyranopolysaccharide. FPSP was in triple-helical structure. The surface of FPSP was a network structure formed by irregular particles. FPSP had both crystalline and amorphous structures. FPSP had good thermostability. CONCLUSIONS ：The optimized extraction technology of FPSP is reasonable ，and has high extraction yield. The research might provide reference for the further development and utilization of F. pallidiflora .
