Study on the Improvement of Quality Standard for Keqing Capsules
- VernacularTitle:咳清胶囊的质量标准提高研究
- Author:
Xue LIU
1
;
Hongyan QIU
2
;
Jing PENG
1
;
Chunyan LIU
3
;
Shuyue QU
3
;
Ying MA
2
;
Qianli XU
1
,
2
;
Xiangchun SHEN
1
;
Ling TAO
1
;
Xiangjun MAO
1
Author Information
1. Guizhou Provincial Engineering Center for High Efficacy Application of Natural Medicinal Resources/Key Laboratory of Natural Medicinal Pharmacology and Druggability Evaluation in Guizhou University/Guizhou Medical University-Guiyang City Joint Key Laboratory/Key Laboratory of Optimal Utilization of Natural Medicine Resources,School of Pharmacy,Guizhou Medical University,Guiyang 550025,China
2. Guizhou Provincial Food and Drug Inspection Institute,Guiyang 550004,China
3. School of Pharmacy,Guizhou University of TCM,Guiyang 550025,China
- Publication Type:Journal Article
- Keywords:
Keqing capsules;
Quality standard;
Morphine;
Codeine phosphate;
TLC;
HPLC
- From:
China Pharmacy
2020;31(5):595-600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimi ze and improve the quality standard for Keqing capsules. METHODS :According to general rule 0502 method stated in 2015 edition of Chinese Pharmacopeia (part Ⅳ),TLC method was used to identify Reineckia carnea and Morus alba in Keqing capsules [the developing solvents were dichloromethane-ethyl acetate-formic acid (10 ∶ 4 ∶ 0.2,V/V/V) and ethyl acetate-carbinol-ammonia (12 ∶ 2 ∶ 1,V/V/V),respectively]. The contents of morphine and codeine phosphate in Keqing capsules were determined by HPLC. The determination was performed on XBridge C 18 column with mobile phase consisted of acetonitrile-0.01 mol/L potassium dihydrogen phosphate aqueous solution (pH value adjusted to 2.7 with 5% phosphoric acid solution)(5 ∶ 95,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm,and the column temperature was 35 ℃. The sample size was 10 µL. RESULTS :In TLC of R. carnea and M. alba in samples ,same color spots were shown in the correspon ding positions of reference substance chromatogram without interference from negative control. The linear range of morphine and codeine phosphate were batches of Keqing capsules were 0.97-1.37,0.16-0.37 mg/g,respectively. CONCLUSIONS :TLC identification method for R. carnea and M. alba ,as well as HPLC content determination method for morphine and codeine phosphate in Keqing capsules are established;the method is simple ,accurate and reliable with strong specificity ,which improves the quality standard of Keqing capsules.