Content Determination of 4 Indicator Components in Shengyu Decoction Lyophilized Powder by HPLC
- VernacularTitle:HPLC法测定圣愈汤冻干粉中4个指标成分的含量
- Author:
Danhua DONG
1
;
Yujun LIU
2
;
Yanan LI
1
;
Xianghao HU
1
;
Ping SUN
1
;
Ting LI
3
;
Juyan LIU
4
;
Peng GAO
1
Author Information
1. School of Pharmacy，Shandong University of TCM，Jinan 250355，China
2. Chinese Pharmacy of Qihe County People’s Hospital of Shandong Province，Shandong Dezhou 251100，China
3. Dept. of Pharmacy，Wendeng Osteopathic Hospital of Shandong Province，Shandong Weihai 264400，China
4. Dept. of Technology，Guangzhou Pharmaceutical Group Co.，Ltd.，Guangzhou 510130，China
- Publication Type:Journal Article
- Keywords:
Shengyu decoction;
Lyophilized powder
- From:
China Pharmacy
2020;31(5):576-580
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish the content determin ation method of ferulic acid ，verbascoside，ligustilide and astragaloside in Shengyu decoction lyophilized powder. METHODS ：HPLC method was adopted to determine 4 components in 3 batches of lyophilized powder. The determination of ferulic acid ，verbascoside and ligustilide was performed on Inertsil ODS-SP C 18 column with mobile phase consisted of methanol- 0.1％ phosphoric acid （gradient elution ）at the flow rate of 1.0 mL/min；detector was diode array detector ；detection wavelength was set at 330 nm；column temperature was 30 ℃，the sample size was 10 μL. The determination of astragaloside was performed on Kromasil C 18 column with mobile phase consisted of acetonitrile-water （32∶68，V/ V）；detector was evaporative light scattering detector ；the drift tube temperature wa s 100 ℃，the carrier gas （air）flow rate was 2.5 L/min at the flow rate of 1.0 mL/min；column temperature was 30 ℃，the sample size was 10 μL. RESULTS：The linear ranges of ferulic acid ，verbascoside，ligustilide and astragaloside were 0.050 15-10.03 μg（r＝0.999 8），0.067 80-13.56 μg（r＝ 0.999 9），0.057 30-11.46 μg（r＝0.999 5），1.128-11.28 μg（r＝0.999 3），respectively. The detection limits were 2.12×10－4，1.30× 10－3，8.02×10－4，1.09×10－3 μg，respectively. The limit of quantification were 7.43×10－4，3.87×10－3，2.34×10－3，3.36×10－3 μg， respectively. RSDs of precision ，stability（12 h）and reproducibility tests were all lower than 2％（n＝6）. Average recovery rates were 99.6％（RSD＝0.83％，n＝6），100.9％（RSD＝1.07％，n＝6），98.8％（RSD＝0.84％，n＝6）and 101.3％（RSD＝0.99％， n＝6），respectively. The contents of ferulic acid ，verbascoside，ligustilide and astragaloside in 3 batches of samples were 1.225-1.248， 0.413-0.424， 0.325-0.332， 0.394-0.404 mg/g， respectively （RSDs among batches were lower than 1.5％ ）. CONCLUSIONS：Established method is stable ，reproducible，rapid and accurate for the content determination of ferulic acid ， verbascoside， ligustilide and astragaloside in Shengyu
