Analysis of Flavonoids Chemical Components in Waste Material during Industrialization of Pueraria thomsonii Resources
- VernacularTitle:粉葛资源产业化过程废弃物中的黄酮类化学成分分析
- Author:
Huiting ZENG
1
;
Chao CHEN
1
;
Huailiang CHU
2
;
Shaoqing ZHU
3
;
Yuanjian YUAN
1
;
Xiaoqing WANG
1
;
Jianhua ZHANG
2
;
Jinbao YU
1
;
Jing LI
1
Author Information
1. Jiangxi Provincial Institute of TCM，Nanchang 330046，China
2. Jiangxi Province Institute of Green and Ecological Pueraria，Jiangxi Shangrao 334000，China
3. Jiangsu Collaborative Innovation Center of TCM Resource Industrialization Process，Nanjing University of Chinese Medicine，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
Pueraria thomsonii;
Waste material;
Flavonoids;
UPLC-Q-TOF-MS;
HPLC;
Structure identification;
Content
- From:
China Pharmacy
2020;31(4):451-456
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the composition and contents of flavonoids chemical components in waste material during industrialization of Pueraria thomsonii resources，and to provide reference for comprehensive development and reasonable utilization of the variety. METHODS ：Using“No. 2 Gange”of P. thomsonii from Jiangxi as objects ，UPLC-Q-TOF-MS and HPLC method were adopted to detect the components and contents of flavonoids in the root （with or without cortex ），cortex，flower， fibrous root ，stem，head and dregs （with or without cortex ）of P. thomsonii as well as dry matter of industrial wastewater （with or without cortex ）after precipitation of pueraria powder. RESULTS ：The linearity ，precision，repeatability，stability and recovery of the established method for content determination of 7 flavonoids（puerarin，daidzin，iridoxine-7-O-xylose glucoside ，genistin， iridin，daidzein and kakkalide ）were all in line with the requirements. Totally 12 kinds of flavonoids were identified ，among which the flavonoids in the root ，cortex，stem，fibrous root ，head and dregs of P. thomsonii as well as dry matter of industrial wastewater were the same ，mainly were puerarin ，daidzin，genistein，daidzein and malonyl-daidzein. The flower of P. thomsonii mainly included iridoxine- 7-O-xylose glucoside ，genistin，iridin，kakkalide，6″-O-xylosyldaidzein，but the components as puerarin ， daidzin and its aglycone were not be detected. The content of puerarin in the head of P. thomsonii was the highest （5.765％）. The contents of puerarin in root and dregs of P. thomsonii as well as dry matter of industrial waste-water in samples with cortex were all higher than in corresponding peeled sample. CONCLUSIONS ：The waste material from the industrialization of P. thomsonii resources contains a lot of flavonoids with rich species and high content ，and can be used as an important raw material for obtaining flavonoids such as puerarin.
