Study on Absorption Characteristics of Gastrodia elata Powder with Different Particle Sizes Based on Rat Everted Intestinal Sac Model in vitro
- VernacularTitle:基于大鼠离体外翻肠囊模型研究不同粒径天麻粉的吸收特性
- Author:
Yan CHEN
1
;
Fan LIU
1
;
Zipeng GONG
1
;
Tingting CHEN
1
;
Tao TAO
2
;
Zhi LIU
3
;
Aimin WANG
1
Author Information
1. Guizhou Provincial Key Laboratory of Pharmaceutics & State Key Laboratory of Functions and Applications of Medicinal Plants & Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM & School of Pharmacy,Guizhou Medical University,Guiyang 550004,China
2. Dept. of Rehabilitation Medicine,Guizhou Provincial People’s Hospital,Guiyang 550002,China
3. Dept. of Pharmacy,the Affiliated Hospital of Guizhou Medical University,Guiyang 550001,China
- Publication Type:Journal Article
- Keywords:
Gastrodia elata;
Particle size;
Everted intestinal sac model;
Absorption characteristics;
Gastrodin;
Balisensides
- From:
China Pharmacy
2020;31(4):413-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compar e the absorpt ion characteristics of gastrodin ,parishin A ,parishin B and parishin C of Gastrodia elata powder,and to explore the effect of particle size on intestinal absorption of above components. METHODS :Based on everted intestinal sac model ,using accumulative absorption amount (Q)and absorption rate constant (Ka)as indexes ,UPLC-MS/MS method was used to determine the absorption of gastrodin ,parishin A ,parishin B and parishin C from different doses (2.5,5,10 g/L) of G. elata powder with different particle sizes (fine powder 146 μm,superfine powder 52 μm,ultrafine powder 37 μm)in different segments(duodenum,jejunum,ileum and colon ). RESULTS :Q and Ka of gastrodin and parishin B (intestinal segment ),Q(colon) and Ka(ileum and colon )of parishin C in 2.5 g/L G. elata superfine powder ;Q and Ka of gastrodin (intestinal segment ),Q and Ka of parishin B (duodenum,jejunum,ileum)and Ka of parishin C (colon)in 2.5 g/L G. elata ultrafine powder ;Q of gastrodin (duodenum),Q of parishin A and parishin B (intestinal segment )and Q of parishin C (duodenum,jejunum)in 5 g/L G. elata superfine powder ;Q(duodenum jejunum ,colon)and Ka(intestinal segment )of gastrodin ,Q of parishin B (duodenum,ileum and colon)and Q of parishin C (duodenum,ileum)in 5 g/L G. elata ultrafine powder ;Q and Ka of parishin B (jejunum,ileum),Q of parishin C (jejunum,ileum)in 10 g/L G. elata superfine powder as well as Q(colon)and Ka(duodenum)of gastrodin ,Q (duodenum,ileum,colon)and Ka(duodenum,colon)of parishin B ,Q(duodenum,ileum)and Ka(duodenum)of parishin C in 10 g/L G. elata ultrafine powder were all increased significantly ,compared with the same dose of G. elata fine powder (P<0.05 or P<0.01). Ka of parishin A (jejunum)and Q of parishin C (duodenum)in 2.5 g/L G. elata superfine powder ;Ka of parishin A (jejunum,ileum), Q and Ka of parishin C (duodenum,jejunum)in 2.5 g/L G. elata ultrafine powder ;Ka of gastrodin (jejunum,ileum and colon ),Ka of parishin A (colon),Ka of parishin B (ileum)and Ka of parishin C (jejunum,ileum)in 5 g/L G. elata superfine powder ;Ka of gastrodin and parishin C (jejunum,ileum and colon ),Q(jejunum,colon)and Ka(colon)of parishin A ,Ka of parishin B (jejunum,ileum)in 5 g/L G. elata ultrafine powder ;Q and Ka of parishin A (ileum)in 10 g/L G. elata superfine powder ;Q(duodenum)and Ka(jejunum) of parishin A ,Ka of parishin C (jejunum)in 10 g/L G. elata ultrafine powder were decreased significantly ,compared with the same dose of G. elata fine powder (P<0.05 or P<0.01). Q of gastrodin (colon),Q(colon)and Ka(ileum,colon)of parishin A ,Q and Ka of parishin B (jejunum,colon),Q and Ka of parishin C (ileum,colon)in 2.5 g/L G. elata fine powder ;Q and Ka of gastrodin (colon),Q(ileum,colon)and Ka(jejunum,ileum,colon)of parishin A ,Ka of parishin C (colon)in 2.5 g/L G. elata superfine powder;Q(colon)and Ka(jejunum,ileum,colon)of parishin A and C ,Q and Ka(ileum,colon)of parishin B in 2.5 g/L G. elata ultrafine powder ;Q and Ka of gastrodin ,parishin A and C (colon),Ka of parishin B (colon)in 5 g/L G. elata fine powder ;Q and Ka of gastrodin and parishin A (colon),Q and Ka of parishin C (jejunum,ileum,colon)in 5 g/L G. elata superfine powder ;Q and Ka of gastrodin(ileum,colon),Q of parishin A (jejunum,ileum,colon),Q and Ka of parishin B (jejunum,colon),Q(jejunum,colon) and Ka(jejunum,ileum,colon)of parishin C in 5 g/L G. elata ultrafine powder ;Q of gastrodin (colon),Q and Ka of parishin A ,B and C (jejunum,ileum,colon)in 10 g/L G. el ata fine powder ;Q of gastrodin (colon),Q and Ka of parishin A and C (jejunum, ileum,colon),Q and Ka of parishin B (colon)in 10 g/L G. elata superfine powder ;Q(colon)and Ka(jejunum,ileum,colon)of gastrodin,Q and Ka of parishin A and C (jejunum,ileum,colon),Q(jejunum,ileum,colon)and Ka(ileum,colon)of parishin B in 10 g/L G. elata ultrafine powder were decreased significantly ,compared with duodeum of the same group (P<0.05). Q and Ka of gastrodin(jejunum)in 2.5 g/L G. elata superfine pow