Determination of Polysaccharide Content ，Cluster Analysis in Acanthopanax senticosus from Different Producing Areas and Optimization of Its Ultrasonic Extraction Technology
- VernacularTitle:不同产地刺五加中多糖含量测定、聚类分析及其超声提取工艺优化
- Author:
Junping LI
1
;
Shenglei GUO
1
,
2
;
Qianbo WANG
3
;
Shipeng FU
1
;
Zhenyue WANG
1
Author Information
1. College of Pharmacy，Heilongjiang University of TCM，Harbin 150040，China
2. Postdoctoral Programme of Heilongjiang ZBD Pharmaceutical Co.，Ltd.，Harbin 150040，China
3. Dept. of Pharmacy，the First Affiliated Hospital of Guangdong Pharmaceuti cal University，Guangzhou 510000，China
- Publication Type:Journal Article
- Keywords:
Acanthopanax senticosus;
Polysaccharide;
Phenol-sulfuric acid method;
Content determination;
Orthogonal test;
Ultrasonic extraction technology;
Cluster analysis
- From:
China Pharmacy
2019;30(18):2541-2545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a determination method for content of polysaccharide in Acanthopanax senticosus， and to conduct cluster analysis and ultrasonic extraction technology optimization. METHODS： The content of polysaccharide in A. senticosus was determined by phenol-sulfuric acid method. Cluster analysis was conducted by using SPSS 23.0 software of the polysaccharide in A. senticosus from 17 habitats. The ultrasonic extraction technology was optimized with L9（34）orthogonal test using extraction temperature， the ratio of material to liquid， extraction time as factors， the content of polysaccharides as index， and then validated. RESULTS： The linear range of glucose ranged from 0.007 75 to 0.151 mg/mL （r＝0.999 1）； the limits of quantification and detection were 2.854， 0.856 μg/mL； RSDs of precision， stability， and repeatability tests were less than 2％； recovery rates of the sample were 98.41％-101.58％（RSD＝1.23％，n＝6）. Cluster analysis results showed that 17 batches of samples could be clustered into three classes； S1， S6， S10， S12 and S13 were clustered into one class； S3-S5 and S7 were clustered into one class； and the rest samples were clustered into one class. The optimal ultrasonic extraction technology was extraction temperature 55 ℃， ratio of material to liquid 1 ∶ 10 （g/mL）， extraction time 35 min. Results of validation tests showed the content of the polysaccharide under the best process condition was 4.36％（RSD＝0.920％，n＝3）. CONCLUSIONS： Established method is simple，reproducible and suitable for determination of polysaccharide in A. senticosus； the optimized ultrasonic extraction technology is stable and feasible.
