Research on the Functional Mechanism of Couplet Medicine of “Bupleuri Radix- Atractylodis macrocephalea Rhizoma”on Mammary Hyperplasia Based on Network Pharmacology
- VernacularTitle:基于网络药理学的“柴胡-白术”药对治疗乳腺增生的作用机制探讨
- Author:
Dailu WU
1
;
Zhexing MAI
2
;
Yi CHEN
2
;
Baoyan CHEN
3
;
Lu ZHANG
2
;
Zhizhong SUN
2
;
Weipeng SUN
2
;
Mei HUANG
1
Author Information
1. Dept. of Galactophore，the First Affiliated Hospital of Guangzhou University of TCM，Guangzhou 510405，China
2. The First Clinical Medical College，Guangzhou University of TCM，Guangzhou 510405，China
3. Dept. of Obstetrics，the First Affiliated Hospital of Guangzhou University of TCM，Guangzhou 510405，China
- Publication Type:Journal Article
- Keywords:
Bupleuri Radix;
Atractylodis macrocephalea Rhizoma;
Couplet medicine;
Mammary hyperplasia;
Network pharmacology;
Target;
Pathway;
Mechanism
- From:
China Pharmacy
2019;30(18):2525-2531
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To screen the active component， target and pathway of couplet medicine of “Bupleuri Radix- Atractylodis macrocephalea Rhizoma”， and to comprehensively explore its potential mechanism. METHODS： Based on the method of network pharmacology， main active componets and potential targets of couplet medicine of “Bupleuri Radix-A. macrocephalea Rhizoma” were retrieved from TCMSP， DRAR-CPI， Genecards and OMIM database. The active component-potential target network and interaction network of potential targets were established by Cytoscape 3.6.0 software. Five potential core targets were screened， and its affinity with active components were validated with molecule docking method. GO classified enrichment analysis and KEGG pathway enrichment analysis of potential targets were carried out to obtain key pathway so as to construct active component-potential target-key pathway network. RESULTS： Totally 17 active components and 47 active component-potential targets were obtained from couplet medicine of “Bupleuri Radix-A. macrocephalea Rhizoma”. Five core targets were obtained， including AKT1， PRKCA， PRKCE， HRas， and PIK3CA. Five signaling pathways were involved， including MAPK pathway， PI3K/AKT pathway， RAS pathway， Estrogen pathway， BMP pathway. CONCLUSIONS： The couplet medicine of “Bupleuri Radix-A. macrocephalea Rhizoma” not only act on multiple targets through multiple components for mammary hyperplasia， but also play a complex network regulation role through the interaction between potential targets.
