Study on the Effects of Prophylactic Administration of Ramulus mori Polysaccharides on Inflammatory Responses of Renal Ischemia Reperfusion Injury Model Mice and Its Mechanism
- VernacularTitle:桑枝多糖预防性给药对肾缺血再灌注损伤模型小鼠炎症反应的影响及机制研究
- Author:
Qian HUANG
1
;
Peihuang LIN
1
;
Dandan ZHENG
1
;
Qiuhong HUANG
1
;
Meiai WANG
1
;
Huiqin CHEN
1
;
Zilu SHI
2
Author Information
1. Teaching and Research Section of Physiology，Basic Medicine Department，Quanzhou Medical College，Fujian Quanzhou 362100，China
2. Dept. of Nephrology，Quanzhou First Hospital of Fujian Medical University，Fujian Quanzhou 362000，China
- Publication Type:Journal Article
- Keywords:
Ramulus?mori?polysaccharides;
Renal ischemia reperfusion injury;
Toll-like receptor 4;
p38 mitogen-activation protein kinase;
IL-6;
IL-10;
Mice
- From:
China Pharmacy
2019;30(13):1786-1791
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study on the effects of prophylactic administration of Ramulus mori polysaccharides （RMP） on inflammatory response of renal ischemia reperfusion injury （RIRI） model mice and to explore its possible mechanism. METHODS： Totally 60 C57BL/6 mice were randomly divided to sham operation group， model group， atorvastatin group （positive control， 15 mg/kg）， RMP low-dose， medium-dose and high-dose groups （300， 600， 1 200 mg/kg）. Except for sham operation group， RIRI model was induced in other 5 groups. 24 h before surgery， they were given relevant medicine intragastrically， once a day， for consecutive one week. 24 h after reperfusion， the mice were sacrificed. The serum levels of Scr and BUN were detected. The morphological changes of renal tissue were observed under optical microscope. The serum levels of IL-1β， IL-6， IL-10 and TNF-α were determined by ELISA. Western blot assay was used to determine the protein expressions of Toll-like receptor 4 （TLR4）， p38 mitogen-activation protein kinase （p38MAPK） and p-p38MAPK. RESULTS： Compared with sham operation group， the serum levels of Scr and BUN were significantly elevated in model group （P＜0.01）. RIRI led to typical inflammatory response of renal tissue， widespread renal tubular epithelial cell degeneration and necrosis， and inflammatory cells infiltration. Serum levels of IL-1β， IL-6， IL-10 and TNF-α were increased significantly （P＜0.01）. The protein expressions of TLR4， p38MAPK and p-p38MAPK were increased significantly in renal cortex （P＜0.01）. Compared with model group， serum levels of Scr and BUN were decreased significantly in administration groups （P＜0.05 or P＜0.01）. The pathological damage of renal tissue was improved in varying degrees， especially in the RMP medium-dose and high-dose groups. Serum levels of IL-1β and IL-6 were decreased significantly in administration groups （P＜0.05 or P＜0.01）. Serum levels of IL-10 were further increased in atorvastatin group and RMP high-dose group （P＜0.01）， and serum level of TNF-α was decreased significantly in atorvastatin group and RMP medium-dose and high-dose groups （P＜0.05 or P＜0.01）. The protein expressions of TLR4 and p-p38MAPK in renal cortex were decreased significantly in administration groups （P＜0.05 or P＜0.01）. CONCLUSIONS： RMP prophylactic administration can improve RIRI of mice， the mechanism of which may be associated with relieving the inflammatory response through inhibition of TLR4/p38MAPK signaling pathway.
