Simultaneous Determination of 6 Flavonoids in the Roots of Tetrastigma hemsleyanum by HPLC
- VernacularTitle:HPLC法同时测定三叶青块根中6个黄酮类成分的含量
- Author:
Caifeng LI
1
,
2
,
3
;
Xin HU
3
;
Pengfei JIN
3
;
Qing LI
1
;
Shuding SUN
1
;
Kaishun BI
1
;
Hongzheng FU
2
Author Information
1. College of Pharmacy，Shenyang Pharmaceutical University，Shenyang 110016，China
2. State Key Lab of Natural and Biomimetic Drugs，Peking University Health Science Center，Beijing 100191，China
3. Dept. of Pharmaceutical Sciences，Beijing Hospital，Beijing 100730，China
- Publication Type:Journal Article
- Keywords:
Tetrastigma hemsleyanum;
Rutin;
Isoquercitrin;
Kaempferol-3-O-rutinoside;
Daunorubicin;
Quercetin;
Kaempferol;
Content determination;
HPLC
- From:
China Pharmacy
2019;30(13):1755-1758
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for simultaneous determination of rutin， isoquercitrin， kaempferol-3-O- rutinoside， daunorubicin， quercetin and kaempferol in the roots of Tetrastigma hemsleyanum. METHODS： HPLC method was adopted. The determination was performed on Alliance SilGreen C18 column with mobile phase consisted of 0.2％ phosphoric acid solution-acetonitrile solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was 360 nm and the column temperature was at 35 ℃. The sample size was 15 μL. RESULTS： The linear range of rutin， isoquercitrin， kaempferol-3- O-rutinoside， daunorubicin， quercetin and kaempferol were 21.77-217.77， 12.37-123.75， 13.23-132.31， 4.63-46.30， 5.75-57.50， 3.36-33.66 μg/mL （all r＝0.999 9）， respectively. limit of detection of them were 0.217 8， 0.123 8， 0.066 2， 0.046 3， 0.191 7， 0.112 3 μg/mL， respectively. limit of quantitation of them were 0.435 6， 0.247 5， 0.165 4， 0.154 3， 0.575 0， 0.421 2 μg/mL， respectively. RSDs of precision （n＝6）， stability （24 h， n＝7） and reproducibility tests （n＝6） were lower than 3.20％. The average recoveries of them were 96.23％， 86.88％， 97.51％， 97.67％， 97.50％， 87.46％， RSDs were 1.85％， 1.90％， 1.84％， 1.87％， 1.25％， 2.01％ （n＝9）， respectively. CONCLUSIONS： The method is fast and simple， and could be applied for simultaneous determination of rutin， isoquercitrin， kaempferol-3-O-rutinoside， daunorubicin， quercetin and kaempferol in the roots of T. hemsleyanum.
