Study on Plasma Protein Binding Rate of Cajanonic Acid A with Different Species of Plasma by Ultrafiltration Combined with UPLC-MS/MS
- VernacularTitle:超滤法结合UPLC-MS/MS法研究树豆酮酸A在不同种属血浆中的血浆蛋白结合率
- Author:
Yujuan BAN
1
;
Li ZHANG
1
;
Rui CHEN
2
;
Gaofeng ZHU
1
,
2
;
Jianta WANG
1
,
2
;
Wenzhang CHEN
2
;
Lei TANG
1
,
2
;
Jing HUANG
1
Author Information
1. School of Pharmacy，Guizhou Medical University，Guiyang 550004，China
2. Guizhou Provincial Engineering Technology Resear ch Cent er for Chemical Drug R&D，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Cajanonic acid A;
Plasma protein binding rate;
Ultrafitration method;
UPLC-MS/MS method;
Rat;
Rabbit;
Human
- From:
China Pharmacy
2019;30(13):1739-1743
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To compare plasma protein binding rate of cajanonic acid A with different species of plasma. METHODS：Using UPLC-MS/MS as the detection means. Plasma protein binding rate of low， medium and high concentrations of cajanonic acid A （2.5， 5， 20 μg/mL） with rats， rabbits and human plasma were determined by ultrafiltration method. The chromatographic conditions included that Waters BEH C18 as chromatographic column， WatersVanGuard BEH C18 as guard column， mobile phase consisted of ultrapure water solution containing 0.01％ formic acid （solvent A） and acetonitrile solution of 0.01％ formic acid （solvent B） gradient elution， at the flow rate of 0.15 mL/min， column temperature of 30 ℃， sample size of 2 μL. Mass spectrum condition included that ESI， negative ion mode acquisition， capillary voltage of 1.5 kV， cone voltage of 30 V， ion source temperature of 100 ℃， desolvent gas temperature of 400 ℃， cone gas flow of 50 L/h， desolvent gas flow of 800 L/h， scanning range of m/z 50→1 200. RESULTS： At the concentration of 2.5， 5 and 20 μg/mL， the plasma protein binding rates of cajanonic acid A were （75.63±0.90）％， （98.30±0.03）％ and （99.42±0.01）％ in the rats plasma； （79.61±1.08）％， （98.48±0.10）％ and （99.42±0.03）％ in rabbits plasma （n＝3）； （76.74±1.22）％， （97.99±0.11）％ and （99.37±0.01）％ in human plasma （n＝3）. At the concentration of 2.5 μg/mL， plasma protein binding rates of cajanonic acid A in plasma of rats and human were significantly lower than that in plasma of rabbits （P＜0.05）. CONCLUSIONS： The plasma protein binding rate of 5，20 μg/mL cajanonic acid A with rats， rabbits and human plasma are higher than that of 2.5 μg/mL cajanonic acid A. There is significant difference in plasma protein binding rate of 2.5 μg/mL cajanonic acid A with different species of plasma，and there is no significant difference in plasma protein binding rate of 5， 20 μg/mL cajanonic acid A with different species of plasma.
