Simultaneous Determination of 8 Non-anthraquinone Constituents in Rheum palmatum by HPLC
- VernacularTitle:HPLC法同时测定大黄药材中8个非蒽醌类成分的含量
- Author:
Wenjin LU
1
;
Zhihua DOU
2
;
Rui CAO
3
;
Lili NI
3
;
Ying DAI
3
Author Information
1. Dept. of Pharmacy，Yixing Second People’s Hospital，Jiangsu Yixing 214221，China
2. Dept. of Pharmacy，Nantong Third Affiliated Hospital of Nantong University，Jiangsu Nantong 226006，China
3. School of Pharmacy，Nanjing University of TCM，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
Rheum palmatum;
Gallic acid;
Catechin;
Epicatechin;
Resveratrol-4′-O-glucopyranoside;
Epicatechin gallate;
Resveratrol-4′-O-β-D-（6″-O-galloyl）-glucopyranoside;
Sennoside A
- From:
China Pharmacy
2019;30(14):1975-1980
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for simultaneous determination of 8 non-anthraquinone constituents in Rheum palmatum. METHODS： HPLC method was adopted. The determination was performed on Symmetry C18 column with mobile phase consisted of methanol-0.1％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃ and detection wavelength was 280 nm. Sample size was 30 μL. RESULTS： The linear range of gallic acid， catechin， epicatechin， resveratrol 4′-O-glucopyranoside， epicatechin gallate， resveratrol 4′-O-β-D-（6″-O-galloyl）-glucopyranoside， sennoside A， 4′-hydroxyphenyl-2-butanone-4′-O-β-D-（2″-O-galloyl-6″-O-p-hydroxy cinnamyl）-glucopyranoside were 6.16-2 464 ng（r＝0.999 9）， 37.4-14 960 ng（r＝0.999 9）， 7.635-3 054 ng（r＝0.999 7）， 7.63-3 052 ng（r＝0.999 9）， 8.32-3 328 ng（r＝0.999 9）， 11.5-4 600 ng（r＝0.999 9）， 16.08-6 432 ng（r＝0.999 9）， 29.3-11 720 ng（r＝0.999 9）， respectively. The limits of quantitation were 3.48， 4.30， 6.40， 4.40， 3.39， 2.87， 8.40 and 4.95 ng， respectively. The limits of detection were 2.32， 2.58， 2.40， 2.64， 2.26， 1.23， 4.20， 2.97 ng， respectively. RSDs of precision， stability and reproducibility tests were all lower than 5％. Recoveries were 94.32％- 100.54％（RSD＝2.78％，n＝6）， 91.15％-99.36％（RSD＝3.72％，n＝6）， 92.16％-98.04％（RSD＝2.39％，n＝6）， 93.41％-100.73％（RSD＝3.17％，n＝6）， 93.89％-98.40％（RSD＝1.99％，n＝6）， 92.61％-101.74％（RSD＝3.71％，n＝6）， 92.66％-103.40％（RSD＝3.76％，n＝6）， 95.45％-102.70％（RSD＝3.06％，n＝6）， respectively. CONCLUSIONS： The established method is simple， accurate and specific， and can be used for the simultaneous determination of 8 non-anthraquinone constituents in R. palmatum.
