Optimization of the Extraction Technology of Verbascoside from Cistanche tubulosa by Box-Behnken Response Surface Me- thodology
- VernacularTitle:Box-Behnken响应面法优化管花肉苁蓉中毛蕊花糖苷的提取工艺研究
- Author:
Zheng KONG
1
;
Lejing MAO
1
;
Shixia HUO
2
;
Ya SU
1
;
Meng JIANG
1
;
Ming YAN
2
Author Information
1. College of Pharmacy，Xinjing Medical University，Urumqi 830011，China
2. Uygur Medicine Research Institute of Xinjiang Uygur Autonomous Region，Urumqi 830049，China
- Publication Type:Journal Article
- Keywords:
Cistanche tubulosa;
Verbascoside;
Response surface methodology;
Box-Behnken design;
Extraction technology;
Optimization
- From:
China Pharmacy
2019;30(14):1970-1974
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To optimize the extraction technology of verbascoside from Cistanche tubulosa， and to provide reference for further development and comprehensive utilization of C. tubulosa. METHODS： The content of verbascoside in C. tubulosa was determined by HPLC. The determination was performed on Inertsil-ODS-3V column with mobile phase consisted of methanol-0.2％ formic acid aqueous solution （40 ∶ 60， V/V） at the flow rate of 1 mL/min. The column temperature was 30 ℃， the detection wavelength was 330 nm， and the sample size was 10 μL. Using extraction rate of verbascoside as index， soaking time， ethanol concentration， liquid-solid ratio， extraction time and extraction times were investigated by single factor tests. According to the results of above tests， ethanol concentration， liquid-solid ratio and extraction time were optimized by Box-Behnken response surface methodology. The verification tests were carried out on the optimized extraction technology. RESULTS： The linear range of verbascoside was 18.65-932.4 μg/mL. The optimal extraction technology included that ethanol concentration 63％， liquid-solid ratio 8 ∶ 1 （mL/g）， soaking for 2 h， extraction time 1.5 h， extracting for 2 times. The extraction rates of verbascoside in the three parallel verification tests were 78.21％， 76.95％， 79.34％， respectively. The relative errors of those to predicted value 76.76％ were 1.89％， 0.25％， 3.36％. CONCLUSIONS： The optimized extraction technology of verbascoside from C. tubulosa is stable and feasible， and is suitable for the extraction of verbascoside.
