Optimization of Purification Technology of Gastrodia elata Total Polyphenols by Macroporous Resin
- VernacularTitle:天麻中总多酚的大孔树脂纯化工艺优化
- Author:
Beibei LIN
1
;
Hongxing ZHENG
1
;
Xiang LIU
1
;
Tianhua ZHOU
1
;
Hongzhong HU
2
;
Chen CHEN
1
Author Information
1. Chinese- German Joint Institute for Natural Product Research，Shaanxi University of Technology/Shaanxi Provincial Engineering Research Center of Gastrodia elata and Cornus officinalis /College of Biological Science and Engineering，Shaanxi University of Technology，Shaanxi Hanzhong 723000，China
2. Shaanxi Baisheng Biological Engineering Co.，Ltd，Shaanxi Lueyang 724300，China
- Publication Type:Journal Article
- Keywords:
Gastrodia elata;
Total polyphenols;
Macroporous resin;
Folin-ciocaileu colorimetry;
Purification technology
- From:
China Pharmacy
2019;30(14):1965-1969
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for content determination of total polyphenols from Gastrodia elata， and to optimize the purification technology of macroporous resin. METHODS： The content of total polyphenols from G. elata was determined by Folin-ciocaileu colorimetry. Using the absorption and desorption performance as index， 4 kinds of macroporous adsorption resins were selected by static adsorption and desorption tests. The flow rate and mass concentration of the sampling solution， volume fraction of eluent， eluent flow rate and eluent volume were investigated by dynamic adsorption and desorption tests. The purification technology of macroporous resin was optimized. RESULTS： The linear range of gallic acid was 4-32 μg/mL （r＝0.999 9）. RSDs of precision， stability and repeatability tests were all less than 2％. The recovery rate of the sample was 95.51％-102.94％（RSD＝2.54％，n＝6）. D301 macroporous resin had strong static adsorption and desorption ability from G. elata polyphenols. The optimal purification technology included that the sample solution flow rate 2 BV/h； the sample solution mass concentration 4 mg/mL； the elution solvent 70％ ethanol； the elution flow rate was 3 BV/h， and the eluent volume 5 BV. The content of total polyphenols from G. elata optimized by the optimal purification technology was 0.381 mg/g. CONCLUSIONS： Established method is sensitive and stable. The optimized purification technology is stable and feasible.
