Optimization of the Extraction Technology of Total Flavonoids from Typhonium divaricatum
- VernacularTitle:山慈菇中总黄酮的提取工艺优化
- Author:
Mengmeng WANG
1
;
Dian XIE
1
;
Yufang ZHOU
2
;
Xiaojing LI
3
;
Bubin XU
1
;
Bin ZHOU
1
Author Information
1. School of Pharmacy，Jiangxi Science and Technology Normal University，Nanchang 330013，China
2. Comprehensive Agriculture Station，Luohe Town People’s Government in Guixi City，Jiangxi Guixi 335400，China
3. Naval Logistics Academy，Tianjin 300450，China
- Publication Type:Journal Article
- Keywords:
Typhonium divaricatum;
Total flavonoids;
Rutin;
Orthogonal test;
Extraction technology;
UV spectrophotometry;
Content determination
- From:
China Pharmacy
2019;30(14):1949-1952
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for the content determination of total flavonoids from Typhonium divaricatum， and to optimize its extraction technology. METHODS： The content of total flavonoids in T. divaricatum was determined by UV spectrophotometry. Using the extraction amount of total flavonoids from T. divaricatum as index， the volume fraction of ethanol， the ratio of material to liquid， the extraction time and times as factors， the extraction technology of total flavonoids from T. divaricatum was optimized by L9（34） orthogonal design， based on the single factor test. RESULTS： The linear range of rutin were 8-48 μg/mL （r＝0.999 7）； the quantification limit was 0.54 μg/mL， and the detection limit was 0.18 μg/mL； RSDs of precision， stability and repeatability tests were all lower than 2％. The recoveries were 99.61％-102.38％（RSD＝1.15％， n＝6）. The optimal extraction technology was as follows： ethanol concentration of 70％， solid-liquid ratio of 1 ∶ 20 （g/mL）， extraction time of 45 min， extracting for 2 times. Under this condition， the average content of total flavonoids from T. divaricatum was 2.74 mg/g. CONCLUSIONS： Established method is simple and accurate； the extraction process is stable and feasible.
