Study on HPLC Fingerprints of Nauclea officinalis Extract Syrup and Content Determination of 9 Components
- VernacularTitle:胆木浸膏糖浆的HPLC指纹图谱研究和9种成分的含量测定
- Author:
Huan LIU
1
;
Xinmiao YU
2
;
Yue WANG
1
;
Jiayue WANG
1
;
Xing LYU
1
;
Lijuan HOU
1
;
Ziwei LIN
1
;
Lixin SUN
1
Author Information
1. College of Pharmacy，Shenyang Pharmaceutical University，Liaoning Benxi 117004，China
2. Grade 2017，Benxi Senior High School，Liaoning Benxi 117000，China
- Publication Type:Journal Article
- Keywords:
Nauclea officinalis extract syrup;
3， 4-dihydroxybenzoic acid;
Neochlorogenic acid;
Loganic acid;
Chlorogenic acid;
Cryptochlorogenic acid;
Swertioside;
Pumiloside;
Strictosamide;
Vincosamide;
HPLC;
Fingerprint;
Content determination
- From:
China Pharmacy
2019;30(14):1940-1945
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish HPLC fingerprints of Nauclea officinalis extract syrup， and to determine the contents of 9 components. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18（2）column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 240 nm， and column temperature was 30 ℃. The sample size was 10 μL. Using strictosamide as reference， HPLC chromatograms of 20 batches of N. officinalis extract syrup were drawn. The similarity of HPLC chromatograms were evaluated by using TCM Fingerprint Similarity Evaluation System （2004A edition） to confirm common peaks. The contents of 9 components were determined by standard curves. RESULTS： There were 26 common peaks in 20 batches of HPLC chromatograms， and the similarity was higher than 0.98. Compared with mixed control， 9 chemical components were identified， such as 3，4-dihydroxybenzoic acid， neochlorogenic acid， loganic acid， chlorogenic acid， cryptochlorogenic acid， swertioside， pumiloside， strictosamide and vincosamide. The linear range of 9 components were 17.24-275.84， 7.56-120.96， 15.40-246.40， 7.84-125.44， 8.64-138.24， 7.96-127.36， 8.40-134.40， 48.56-776.96， 4.16-66.56 μg/mL（all r≥0. 999）， respectively. The limits of detection were 0.043 1， 0.126 0， 0.038 5， 0.130 7， 0.144 0， 0.066 3， 0.070 0， 0.012 1， 0.052 0 μg/mL， respectively. The limits of quantitation were 0.215 5， 0.189 0， 0.077 0， 0.196 0， 0.288 0， 0.132 7， 0.105 0， 0.097 6， 0.138 7 μg/mL， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％ （n＝6）. Average recoveries were 99.6％、106.3％、100.1％、102.0％、98.4％、100.0％、99.3％、100.6％ and 101.2％， and RSDs were 1.20％、0.24％、0.59％、1.00％、0.73％、1.30％、1.10％、1.80％、1.90％（n＝6）. CONCLUSIONS： Established HPLC fingerprints and quantitative determination method of N. officinalis extract syrup are accurate， specific and sensitive. It can provides reference for quality control of N. officinalis extract syrup.
