Quantitative Determination of Two Flavonoids and Four Phenoquinones of Huoxue Cuyu Capsules by HPLC
- VernacularTitle:HPLC法同时测定活血促愈胶囊中2种黄酮类和4种菲醌类成分的含量
- Author:
Dan CHEN
1
;
Ke LI
2
;
Maofang LU
3
;
Xi HOU
2
;
Ruocun LI
2
Author Information
1. Xiangtan Medical and Health Vocational and Technical College，Hunan Xiangtan 411102，China
2. Hunan Academy of TCM，Changsha 410013，China
3. School of Pharmacy，Hunan University of TCM，Changsha 410208，China
- Publication Type:Journal Article
- Keywords:
Huoxue cuyu capsules;
Rutin;
Kaempferol-3-O-rutinoside;
Dihydrotanshinone Ⅰ;
Cryptotanshinone;
Tanshinone Ⅰ;
Tanshinone ⅡA;
HPLC
- From:
China Pharmacy
2019;30(14):1931-1935
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish a method for simultaneous determination of two flavonoids （rutin and kaempferol-3-O- rutinoside） and four phenoquinones （dihydrotanshinone Ⅰ，cryptotanshinone，tanshinone Ⅰ and tanshinoneⅡA） in Huoxue cuyu capsules. METHODS：HPLC method was adopted. The determination was performed on Welch Ultimate XB-C18 column with mobile phase consisted of acetonitrile-0.1％phosphoric acid solution（gradient elution）at the flow rate of 1.0 mL/min. The column temperature was set at 20 ℃，and detection wavelength was set at 270 nm. The sample size was 10 μL. RESULTS：The linear range of rutin，kaempferol-3-O-rutinoside，dihydrotanshinone Ⅰ，cryptotanshinone，tanshinone Ⅰ and tanshinoneⅡA were 172.13-860.66 μg/mL（r＝0.999 7），15.33-76.66 μg/mL（r＝0.999 8），12.81-64.06 μg/mL（r＝0.999 3），5.90-29.52 μg/mL（r＝0.999 3），5.12-25.60 μg/mL（r＝0.999 2），6.71-33.57 μg/mL（r＝0.999 7），respectively. The limits of detection were 0.08，0.01，0.01，0.01，0.01，0.01 μg/mL. The limits of quantitation were 0.27，0.02，0.03，0.03，0.03，0.03 μg/mL，respectively. RSDs of precision，stability test and repetition tests were all lower than 2.0％（n＝6）. The recoveries were 97.54％-100.25％（RSD＝1.07％，n＝6），96.90％-101.91％（RSD＝1.73％，n＝6），96.24％-102.89％（RSD＝2.32％，n＝6），97.04％-102.18％（RSD＝1.82％，n＝6），95.06％-97.73％（RSD＝1.18％，n＝6），95.59％-101.40％（RSD＝2.29％，n＝6），respectively. CONCLUSIONS：The method is sensitive，rapid，simple and has good reproducibility. It can be used for simultaneous determination of rutin， kaempferol-3-O-rutinoside， dihydrotanshinone Ⅰ， cryptotanshinone， tanshinone Ⅰ， tanshinoneⅡA in Huoxue cuyu capsules.
