Study on Chemical Constituents of n-Butanol Part from the Roots of Camellia oleifera
- VernacularTitle:油茶根正丁醇萃取部位的化学成分研究
- Author:
Fenghua WANG
1
;
Qi REN
2
;
Shourong HU
2
;
Huizheng FU
2
Author Information
1. Dept. of Pharmacy，the Fourth Affiliated Hospital of Nanchang University，Nanchang 330003，China
2. Jiangxi Provincial Institute for Drug Control/Jiangxi Provincial Engineering Research Center for Drug and Medical Device Q uality，Nanchang 330029，China
- Publication Type:Journal Article
- Keywords:
Camellia oleifera;
n-Butanol part;
Chemical constituents;
Separate;
Identify
- From:
China Pharmacy
2019;30(17):2369-2373
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study the chemical constituents of n-Butanol part from the roots of Camellia oleifera， and to provide reference for elucidating the pharmacodynamic substance basis of it’s anti-tumor effect. METHODS： The ethanol extracts were obtained by using 95％ ethanol as extraction solvent to extract the roots of C. oleifera， and the n-Butanol part was obatined after the extracts were extratced with ethyl acetate and water-saturated n-butanol solution in turn. The chemical constituents of n-butanol part were isolated and purified by D101 macroporous resin column， silica gel， atmospheric pressure reversed phase column chromatography， sephadex gel SephadexLH-20 column chromatogram and preparative HPLC. The structure of the compounds was identified by spectroscopic analysis of physicochemical constants， electrospray ionization mass spectrometry， 1H-NMR and 13C-NMR. RESULTS： Eight compounds were isolated from the roots of C. oleifera and elucidated as quercetin 3′ -O-β-D-glucoside （compound 1）， apigenin-7-O-β-D-glucoside （compound 2）， （+）-lyoniresinol-3α-O-β-D-glucopyranoside （compound 3）， rubusoside （compound 4）， dulcoside B （compound 5）， 4-hydroxy-3-methoxyphenol 1-O-β-D-[6-O-（4-hydroxy-3，5-dimethoxylbenzoate）-glucopyranoside （compound 6）， 3，4，5-trimethoxyphenyl-6-O- syringoyl-β-D-glucopyranoside （compound 7）， gordonoside P （compound 8）. CONCLUSIONS： Compounds 1-8 were isolated from this plant for the first time. This not only enriches the chemical constituents of this genus， but also provides a reference for elucidating the anti-tumor bioactive substances in this part.
