Rapid Analysis of Chemical Components of Zhitong Huazheng Capsules Based on UPLC-Q-TOF/MS Technology Combined with UNIFI Database
- VernacularTitle:UPLC-Q-TOF/MS技术结合UNIFI数据库快速分析止痛化癥胶囊的化学成分
- Author:
Fulin WU
1
;
Cuizhu WANG
1
;
Qinghai DONG
1
;
Han WANG
1
;
Jing TAN
1
;
Hongqiang LIN
1
;
Jinping LIU
1
;
Pingya LI
1
Author Information
1. School of Pharmacy，Jilin University，Changchun 130021，China
- Publication Type:Journal Article
- Keywords:
Zhitong huazheng capsules;
Chemical components;
UNIFI database;
UPLC-Q-TOF/MS
- From:
China Pharmacy
2019;30(17):2336-2342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To rapidly identify chemical components of Zhitong huazheng capsules （shorted for “ZTHZC”）， and to provide reference for further elucidating the pharmacodynamic material basis and overall quality control. METHODS： UPLC-Q-TOF/MS method was adopted to separate the chemical components of ZTHZC. By reviewing literatures that related to chemical components of ZTHZC and its single Chinese traditional medicinal crops， the chemical structure of the chemical components was saved into a .mol format file， and supplemented the UNIFI database. The chemical constituents of ZTHZC were qualitatively analyzed by UNIFI database screening， and validated according to their precise molecular mass， characteristic ions， neutral loss， secondary mass spectrometry cleavage， chromatographic retention behavior， combined reference information and literature reports. RESULTS： A total of 70 chemical components were identified in this experiment， including 38 organic acids and organic acid esters， 8 alkaloids， 9 flavonoids， 5 triterpenoid saponins， 5 aldehydes， 2 ketones， 1 quinone， 1 polyacetylene and 1 monoterpenoid. CONCLUSIONS： This study provides a rapid detection and identification method for chemical components of ZTHZC， and also provides a scientific basis for the clarification of ZTHZC’s quality control and pharmacodynamics.
