Content Determination of 6 Flavonoids in Epimedium brevicornu from Shenqi Yanshen Granules Based on HPLC-QAMS
- VernacularTitle:HPLC-一测多评法测定参芪延肾颗粒中淫羊藿药材中的6个黄酮类成分的含量
- Author:
Jie SHEN
1
;
Qin WANG
1
;
Weijian XIONG
1
;
Chong XU
1
Author Information
1. Dept. of Pharmacy,Chongqing Hospital of TCM,Chongqing 400021,China
- Publication Type:Journal Article
- Keywords:
Shenqi yanshen granules;
Epimedium brevicornu;
Icariin;
Flavonoid;
QAMS;
HPLC;
Content determination;
Quality control
- From:
China Pharmacy
2019;30(17):2327-2331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for simultaneous determination of 6 flavonoids in Epimedium brevicornu from Shenqi yanshen granules, such as epimedin A1, epimedin A, epimedin B, epimedin C, icariin and baohuoside Ⅰ. METHODS: HPLC method was adopted. The determination was performed on Waters Symmetry C18 column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min; the column temperature was 25 ℃, and detection wavelength was 270 nm. The sample size was 10 μL. Relative correction factors (fk/s) of each component to icariin (reference substance) were established by multi-point correction method and slope correction method on the basis of external standard method to calculate the contents of each component. The contents of 6 flavonoids in 4 batches of Shenqi yanshen granules determined by HPLC external standard method were compared with by multi-point correction method and slope correction method. Feasibility and accuracy of quantitative analysis of multi-components by single-marker (QAMS) were validated. RESULTS: The linear range of epimedin A1, epimedin A, epimedin B, epimedin C, icariin and baohuoside Ⅰ were 2.03-50.80 μg/mL (r=0.999 5), 4.34-108.60 μg/mL (r=0.999 5), 2.26-56.40 μg/mL (r=0.999 5), 4.14-103.60 μg/mL (r=0.999 5), 4.24-106.00 μg/mL (r=0.999 5), 1.78-44.60 μg/mL (r=0.999 5), respectively, the limits of detection were 65.80, 71.49, 74.26, 68.79, 70.56, 86.09 ng/mL, respectively; the limits of quantification were 196.62, 213.63, 223.72, 208.46, 215.96, 255.88 ng/mL, respectively; RSDs of precision, stability (24 h), reproducibility tests were less than 2% (n=6), respectively. The average recoveries were 96.03%-99.04% (RSDs were 0.65%-1.04%, n=6). By multi-point correction method, fk/s of epimedin A1, epimedin A, epimedin B, epimedin C and baohuoside Ⅰ were 0.837, 0.818, 0.845, 0.831, 1.387, respectively; by slope correction method, fk/s of them were 0.835, 0.815, 0.851, 0.829, 1.419, respectively. There was no significant difference in content determination results between two correction methods of QAMS and external standard (P>0.05). CONCLUSIONS: Established HPLC- QAMS method is accurate and suitable for the quality control of epimedin A1, epimedin A, epimedin B, epimedin C, icariin and baohuoside Ⅰ in E. brevicornu from Shenqi yanshen granules.