Simultaneous Determination of the Contents of 6 Volatile Components in Zhachong Shisan by GC
- VernacularTitle:气相色谱法同时测定扎冲十三药材中6种挥发性成分的含量
- Author:
Caijing YANG
1
;
Renli CAO
1
;
Ying ZHAO
1
;
Xiaofeng WANG
1
;
Xun LIU
2
;
Yanli GU
1
Author Information
1. College of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China
2. Dept. of Medicine and Food，Tongliao Vocational College，Inner Mongolia Tongliao 028000，China
- Publication Type:Journal Article
- Keywords:
Zhachong shisan;
Gas chromatography;
Content determination;
Volatile component
- From:
China Pharmacy
2019;30(12):1650-1654
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for simultaneously determining the contents of 6 volatile components such as eugenol， methyl eugenol， isoeugenol， α-asarone， alkaloid and myristyl ether in Zhachong shisan. METHODS：Volatile oil was extracted by steam distillation and analyzed by gas chromatography. The chromatographic column was ov-1701， with hydrogen flame ionization detector. The carrier gas was nitrogen， the inlet temperature was 220 ℃， the detector temperature was 240 ℃， the flow rate was 1.0 mL/min， the injection volume was 1 μL， the split ratio was 10 ∶ 1， with programmed temperature. RESULTS： The linear ranges of eugenol， methyl eugenol， isoeugenol， α-asarone， costunolide and myristyl were 2.00-12.00， 1.00-6.00， 0.60-3.60， 0.05-0.30， 0.05-0.30， 0.60-3.60 mg/mL（r＝0.999 9）. The detection limits were 0.01， 0.01， 0.02， 0.01， 0.01， 0.01 mg/mL， respectively； the limits of quantification were 0.05， 0.03， 0.06， 0.02， 0.02， 0.03 mg/mL， respectively. RSDs of precision， stability and reproducible tests were all lower than 3％； average recovery rates were 99.33％-100.04％， RSDs were 0.02％-0.35％ （n＝6）； RSDs of durability test were all lower than 5％. CONCLUSIONS： The established method was simple and reproducible. It can be used for simultaneous determination of the contents of 6 volatile components in Zhachong shisan.
