Isolation and Identification of the Coumarins from the Seeds of Clausena lansium and Study on Their Inhibitory Activity of α-glucosidase and Nematicidal Activity against Panagrellus redivivus
- VernacularTitle:黄皮种子中香豆素类化合物的分离、鉴定及其α-葡萄糖苷酶抑制活性和全齿复活线虫致死活性研究
- Author:
Shuhui XU
1
;
Huiqin CHEN
2
,
3
;
Yujiao FAN
2
,
3
;
Pengwei CHEN
2
,
3
;
Wenli MEI
2
,
3
;
Haofu DAI
2
Author Information
1. Dept. of Pharmacy，the Affiliated Haikou Hospital of Xiangya Medical College，Central South University，Haikou 570208，China
2. Key Lab of Biology and Genetic Resources of Tropical Crops，Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology，Chinese Academy of Tropical Agricultural Sciences，Haikou 571101，China
3. Hainan Key Lab for Research and Utilizatio n of Li Medicine Natural Products，Haikou 571101，China
- Publication Type:Journal Article
- Keywords:
Clausena lansium;
Seeds;
Coumarins;
Isolation;
Identification;
α-glucosidase;
Panagrellus redivivus
- From:
China Pharmacy
2019;30(4):518-522
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To isolate and identify the coumarins from the seeds of Clausena lansium， and to study their inhibitory activity of α-glucosidase and nematicidal activity against Panagrellus redivivus. METHODS： Column chromatography， reversed phase silica gel column chromatography and HPLC method were used to separate and purify the coumarins from the seeds of C. lansium. The structures of compounds were identified according to physicochemical properties， 1H-NMR and 13C-NMR spectral data. Using acarbose and avermectin as positive control， PNPG and Berman funnel methods were used to investigate the α-glucosidase inhibitory activity and nematicidal activity against P. redivivus， respectively. RESULTS： Seven coumarins compounds were isolated from the seeds of C. lansium， and were identified as 7-hydroxy-1-benzopiran-2-one （Ⅰ）， Wampetin （Ⅱ）， Lansiumarin-C （Ⅲ）， Claucoumarin A （Ⅳ）， Clausenalansimin A （Ⅴ）， （E，E）-8-（7-hydroxy-3，7-dimethylocta-2，5-dienyloxy） psoralen （Ⅵ）， Dihydroindicolactone （Ⅶ）. Under 0.25 mg/mL， the α-glucosidase inhibitory rates of compounds Ⅰ， Ⅲ， Ⅴ were （32.4±1.9）％，（37.1±6.0）％， （39.5±1.1）％， respectively. Under 2.5 mg/mL， corrected mortality of compounds Ⅰ， Ⅳwere 50.5％ and 47.9％. CONCLUSIONS： Compounds Ⅰ， Ⅲ， Ⅴ show α-glucosidase inhibitory activity， and compounds Ⅰ，Ⅳ display nematicidal activity against P. redivivus. α-Glucosidase inhibitory activity of compounds Ⅲ， Ⅴ， and nematicidal activity of compound Ⅳ are found for the first time.
