Simultaneous Determination of 6 Components in Libiling Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法同时测定痢必灵片中6种成分的含量
- Author:
Yang XU
1
;
Baiyu SHAN
2
;
Huiwei BAO
3
Author Information
1. College of Drug and Food,Changchun Medical College,Changchun 130031,China
2. Baicheng Institute for Food and Drug Control,Jilin Baicheng 137000,China
3. College of Pharmacy,Changchun University of TCM,Changchun 130117,China
- Publication Type:Journal Article
- Keywords:
Libiling tablets;
RP-HPLC;
Matrine;
Oxymatrine;
Gallic acid;
Peoniflorin;
Costunolide;
Dehydrocostus lactone;
Content determination
- From:
China Pharmacy
2019;30(4):454-457
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for simultaneous determination of matrine, oxymatrine, gallic acid, peoniflorin, costunolide and dehydrocostus lactone in Libiling tablets. METHODS: RP-HPLC method was adopted. The determination was performed on Agilent ZORBAX SB-C18 column with mobile phase consisted of methanol-0.1% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelengths were 210 nm (matrine, oxymatrine) and 225 nm (gallic acid, peoniflorin, costunolide, dehydrocostus lactone). The column temperature was set at 30 ℃, and sample size was 5 μL. RESULTS: The linear ranges of matrine, oxymatrine, gallic acid, peoniflorin, costunolide, dehydrocostus lactone were 0.053-5.28 mg/mL(r=0.999 8), 0.125-12.54 mg/mL(r=0.999 9), 0.013-1.33 mg/mL(r=0.999 8), 0.169-16.94 mg/mL(r=0.999 9), 0.048-4.77 mg/mL(r=0.999 8), 0.072-7.16 mg/mL (r=0.999 9). The limits of quantitation were 4.08×10-4, 4.48×10-4, 3.12×10-4, 2.10×10-4, 1.36×10-4, 1.84×10-4 mg/mL, respectively. The limits of detection were 1.24×10-4, 1.50×10-4, 1.02×10-4, 6.20×10-5, 4.20×10-5, 6.40×10-5 mg/mL, respectively. RSDs of precision, stability and reproducibility tests were all lower than 2% (n=6). The recoveries were 98.03%-101.43% (RSD=1.25%, n=6), 97.73%-102.26% (RSD=1.96%, n=6), 97.18%-101.41% (RSD=1.98%,n=6), 97.45%-102.11% (RSD=1.88%,n=6), 96.85%-101.07% (RSD=1.75%, n=6), 97.12%-102.64% (RSD=1.82%,n=6), respectively. CONCLUSIONS: Established method is simple, stable and rapid, and can be used for simultaneous determination of 6 components in Libiling tablets.