Isolation and Identification the Compositions of Alkaloids in Acorus tatarinowii
- VernacularTitle:石菖蒲中生物碱成分的分离与鉴定
- Author:
Xiaoling HU
1
;
Yi TANG
1
;
Jinbin YUAN
2
Author Information
1. Dept. of Pharmacy,Jiangxi Hospital of Integrated Traditional and Western Medicine,Nanchang 330003,China
2. College of Pharmacy,Jiangxi University of TCM,Nanchang 330000,China
- Publication Type:Journal Article
- Keywords:
Acorus tatarinowii;
Alkaloids;
UPLC-MS;
Isolation;
Identification
- From:
China Pharmacy
2019;30(5):642-645
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To isolate and identify the compositions of alkaloids in Acorus tatarinowii, and to lay the foundation for further study of its pharmacological activity. METHODS: The compositions of alkaloids from A. tatarinowii was separated by cation exchange resin. UPLC-MS method was used to identify the alkaloids. The separation was performed on a Agilent Zorbax Eclipse Plus-C18 column with mobile phase consisted of 0.1% formic acid-acetonitrile (gradient elution) at a flow rate of 0.3 mL/min. The column temperature was 30 ℃, and the injection volume was 1 μL. ESI ion source was used to detect the fragments, the ion mode scan was positive. Peakview 1.2 and other softwares were used to analyze the compositions of alkaloids. RESULTS: 86 compositions were separated and 5 alkaloids were identified, such as tatarine A, N-trans-feruloyltyramine, N-lauryldiethanolamine, phytosphingosine and 5-butyluridine. CONCLUSIONS: The results of the study lay a foundation for the study of pharmacological activity of alkaloids from A. tatarinowii.