Separation and Identification of Chemical Components in Ethyl Acetate Fraction and Water Fraction from Tripterygium wilfordii
- VernacularTitle:雷公藤乙酸乙酯部位和水部位化学成分的分离与鉴定
- Author:
Ruikun YANG
1
;
Sifang WU
2
;
Jun YAN
1
;
Jicheng SHU
1
;
Rui ZHANG
1
;
Shenglin ZHANG
1
;
Tianyou CAO
1
;
Jianqun LIU
1
Author Information
1. Key Lab of Modern Chinese Medicine Preparation Minisery of Education,Jiangxi University of TCM,Nanchang 330004,China
2. East Street Community Health Service Center,Pingxiang Municipal Second People’s Hospital,Jiangxi Pingxiang 337000,China
- Publication Type:Journal Article
- Keywords:
Tripterygium wilfordii;
Separation;
Identification;
HPLC;
Glucosides compound
- From:
China Pharmacy
2019;30(5):638-641
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To separate and identify chemical components in ethyl acetate fraction and water fraction from Tripterygium wilfordii, and to provider basis for further pharmacological study. METHODS: The ethyl acetate fraction and water fraction from T. wilfordii were separated and purified by MCI GEL-CHP 20P column chromatography, C18 RP silica gel column chromatography, Sephadex LH-20 gel column chromatography and HPLC. The structures of compounds were analyzed and identified by 1H-NMR, 13C-NMR and physicochemical properties. RESULTS: Two compounds were isolated from ethyl acetate fraction of T. wilfordii, namely orthosphenic acid (compound 1), dibutylphthalate (compound 2). Eight glucosides were isolated from water extract of T. wilfordii, namely 2,6-dimethoxy-4-hydroxymethyl-phenyl-1-O-beta-D-glucopyranoside (compound 3), 2,6-dimethoxy-4-hydroxyphenol-1-O-β-D-glucoside(compound 4), 4-hydroxy-1-(2-hydroxyethyl)-phenyl-3-O-β-D-glucopyranoside (compound 5), 3,4-dimethoxy-phenyl-1-O-β-D-glucopyranoside (compound 6),β-adenosine (compound 7), ligustrin (compound 8), epicatechin-8-C-β-D-galactoside (compound 9) and 2-hydroxynaringenin-7-O-β-glucoside (compound 10). CONCLUSIONS: Chemical components of ethyl acetate fraction and water fraction are separated and identified from T. wilfordii.