Study on Improvement Effect of Methylated Urolithin A on Oleic Acid-induced Lipid Accumulation in Huh- 7 Cells and Its Me- chanism
- VernacularTitle:甲基尿石素A对油酸诱导的Huh-7细胞脂质累积的改善作用及其机制研究
- Author:
Cong ZHANG
1
;
Junxuan ZHOU
1
;
Lei SHENG
1
;
Junqiao MA
1
;
Xin LI
1
;
Guohua ZHENG
1
;
Sidan LIU
1
;
Zhengpeng QIU
1
Author Information
1. College of Pharmacy，Hubei University of TCM，Wuhan 430065，China
- Publication Type:Journal Article
- Keywords:
Methylated urolithin A;
Oleic acid;
Human liver cancer Huh-7 cells;
Lipid accumulation;
Mechanism
- From:
China Pharmacy
2019;30(6):741-746
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study the improvement effect and mechanism of methylated urolithin A on oleic acid-induced lipid accumulation in human liver cancer Huh-7 cells. METHODS： Oleic acid was adopted to induce lipid accumulation model cells. Huh-7 cells were divided into control group （culture medium）， model group （1 mmol/L oleic acid）， low-dose group （1 mmol/L oleic acid+10 μmol/L methylated urolithin A） and high-dose group （1 mmol/L oleic acid+20 μmol/L methylated urolithin A）. Oil red O staining was used to observe lipid accumulation in cells. Triglyceride（TG） enzyme assay was applied to determine the TG content in cells. PCR was employed to detect the mRNA expression of FASN， SREBP-1， PPAR-α and PPAR-γ in cells. Western blotting was used to determine the protein expression of FASN in cells. RESULTS： After induced by oleic acid， a large amount of lipid droplet accumulated around the cells； the intracellular lipid and TG content， mRNA expression levels of FASN， SREBP-1 and PPAR-γ， protein expression levels of FASN were increased significantly， while mRNA expression level of PPAR-α was decreased significantly （P＜0.01）. After intervened with methylated urolithin A， lipid droplet around the cells decreased significantly； the contents of lipid and TG in cells were decreased significantly， while the mRNA expression levels of FASN， SREBP-1 and PPARγ and protein expression level of FASN were decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS： Methylated urolithin A can improve oleic acid-induced lipid accumulation in Huh-7 cells， the mechanism of which may be associated with inhibiting fat synthesis， promoting lipid metabolism and down-regulating the expression of metabolism-related factors as FASN， SREBP-1 and PPAR-γ.
