Study on Prevention Effect and Mechanism of Miao Medicine Liangjiang Weiyang Capsule on Gastric Ulcer Model Rats
- VernacularTitle:苗药良姜胃疡胶囊对胃溃疡模型大鼠的预防作用及机制研究
- Author:
Lianli DENG
1
;
Jinjuan ZHANG
2
;
Bing LIANG
2
;
Ying XIONG
2
;
Shanggao LIAO
1
,
3
;
Haibo WANG
4
;
Xun HE
3
Author Information
1. School of Pharmacy，Guizhou Medical University，Guiyang 550025，China
2. School of Basic Medicine，Guizhou Medical University，Guiyang 550025，China
3. Guizhou Provincial Key Laboratory of Pharmaccutics，Guiyang 550004，China
4. Sunflower Pharmaceutical Group （Guizhou）Hongqi Co.，Ltd.，Guizhou Liupanshui 553400，China
- Publication Type:Journal Article
- Keywords:
Miao medicine;
Liangjiang weiyang capsule;
Gastric ulcer;
Pepsin activity;
NF-κB;
Rats
- From:
China Pharmacy
2019;30(7):946-950
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study the protective effect and mechanism of Miao medicine Liangjiang weiyang capsule on gastric ulcer model rats. METHODS： Rats were randomly divided into normal group （normal saline）， model group （normal saline）， positive control group （omeprazole， 0.02 g/kg） and Liangjiang weiyang capsule low-dose， medium-dose and high-dose groups （0.3， 0.6， 1.2 g/kg）， with 12 rats in each group. All rats were intragastrically administered once a day for consecutive one week. 1 h after last administration， all rats except those in normal group were given the absolute ethanol to induce gastric ulcer model. 1 h after modeling， gastric juice volume， gastric juice pH， pepsin activity， gastric ulcer area and inhibitory rate of gastric ulcer were recorded in each group. Histopathological changes of gastric mucosa in rats of each group were observed by HE staining. The serum levels of TNF-α and IL-6 were determined by ELISA. The expression of nuclear factor-κB pathway related protein （p-NF-κB p65， p-IκBα） in gastric tissue of rats were determined by Western blot. RESULTS： Compared with normal group， gastric juice volume， pepsin activity， gastric ulcer area， TNF-α and IL-6 levels in serum， p-NF-κB p65 and p-IκBα levels in the gastric tissue were significantly increased/rised （P＜0.05）， while gastric juice pH was significantly decreased （P＜0.01）； there were gastric mucosal hyperemia and redness， obvious defect of mucosal epithelial cells， destruction of gland structure and incomplete cell structure. Compared with model group， gastric juice volume， pepsin activity， gastric ulcer area and the levels of TNF-α and IL-6 were reduced/decreased significantly in positive control group， Liangjiang weiyang capsule medium-dose and high-dose groups （P＜0.05 or P＜0.01）， while pH value of gastric juice was increased significantly （P＜0.05）； gastric mucosa was normal， gland destruction was alleviated and cell structure was intact. The levels of p-NF-κB p65 and p-IκBα in gastric tissue were significantly decreased in Liangjiang weiyang capsule high-dose groups （P＜0.05）. CONCLUSIONS： Liangjiang weiyang capsule play a role to protect gastric ulcer by increasing gastric juice pH， inhibiting pepsin activity， reducing the release of inflammatory factors as TNF-α， IL-6 and inhibiting the expression of NF-κB pathway related protein.
