Preparation and Characterization of Syringopicroside Solid Lipid Nanoparticles
- VernacularTitle:丁香苦苷固体脂质纳米粒的制备及表征
- Author:
Xiwu ZHANG
1
;
Qiuhan LI
1
;
Yingpeng LI
2
;
Yongji LI
3
;
Zuodi XU
1
;
Jinjin DOU
4
Author Information
1. Academy of TCM，Heilongjiang University of TCM，Harbin 150040，China
2. College of TCM，Tianjin University of TCM，Tianjin 301617，China
3. College of Pharmacy，Heilongjiang University of TCM，Harbin 150040，China
4. College of Clinical Medicine，Heilongjiang University of TCM，Harbin 150040，China
- Publication Type:Journal Article
- Keywords:
Syringopicroside;
Solid lipid nanoparticle;
Preparation;
Formula technology;
Characterization
- From:
China Pharmacy
2019;30(9):1168-1172
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To prepare Syringopicroside solid lipid nanoparticles （SYR-SLN）， and optimize the formula and characterize SYR-SLN. METHODS： SYR-SLN were prepared by emulsion evaporation method. Using entrapment efficiency as index， based on single factor， orthogonal design was adopted to optimize the mass ratio of lecithin-monoglyceride， volume ratio of organ phase to water phase， poloxamer 188 （F68） concentration and drug dosage. The optimal formula technology was established to investigate entrapment efficiency， drug-loading amount， morphology， particle size， Zeta potential， stability， etc. RESULTS： The mass ratio of lecithin-monoglyceride was 3 ∶ 1； the volume ratio of organic phase to water phase was 1 ∶ 2； the concentration of F68 was 0.4％； drug dosage was 10 mg. The optimal formula included that monoglyceride 80 mg， lecithin 240 mg， 0.4％ F68， syringopicroside 10 mg， absolute ethyl alcohol 5 mL， distilled water 10 mL， emulsification temperature at 65℃ and stirring at 600 r/min. Encapsulation efficiency of SYR-SLN was （42.35±0.60）％ （n＝3）； drug-loading amount was （5.33±0.03）％ （n＝3）； SYR-SLN had a spherical morphology and was evenly distributed. The average particle size was （180.30±5.31） nm with Zeta potential of （－41.9±0.8） mV， and the SYR-SLN could maintain stable for 15 days at 4℃. CONCLUSIONS： SYR-SLN is prepared successfully， and the technology is simple with high encapsulation efficiency.
