Simultaneous Determination of Isoquercitrin ,Astragalin and Salvianolic Acid B in Moringa oleifera Leaves Granules by HPLC
- VernacularTitle:HPLC法同时测定辣木叶颗粒中异槲皮苷、紫云英苷和丹酚酸B的含量
- Author:
Haiyang YANG
1
;
Yang LIU
1
;
Mengwei LI
1
;
Xueyan LI
1
;
Jun HE
2
;
Wenhong GU
2
;
Jiyun YIN
2
Author Information
1. College of TCM,Beijing University of TCM,Beijing 102488,China
2. Yunnan Tianyou Technology Co.,Ltd.,Yunnan Mangshi 678400,China
- Publication Type:Journal Article
- Keywords:
Moringa oleifera leaves granules;
HPLC;
Isoquercitrin;
Astragalin;
Salvianolic acid B;
Content determination
- From:
China Pharmacy
2019;30(9):1164-1167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for simultaneous determination of isoquercitrin, astragalin and salvianolic acid B in Moringa oleifera leaves granules. METHODS: HPLC method was adopted. The determination was performed on Cosmosil-C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (gradient elution) at flow rate of 1.3 mL/min. The column temperature was 40 ℃ and detection wavelength was set at 260 nm. The sample size was 10 μL. RESULTS: The linear ranges of isoquercitrin, astragalin and salvianolic acid B were 0.017-0.341, 0.010-0.194, 0.010-0.195 mg/mL, respectively (all r>0.999 0). The detection limits were 0.085, 0.143, 0.117 μg/mL, and the limits of quantitation were 0.283, 0.476, 0.392 μg/mL, respectively. RSDs of precision, stability (24 h) and reproducibility tests were all lower than 2.0% (n=6). Average recoveries were 101.22%, 98.76% and 98.72%, and RSDs were 0.66%,0.30%,0.30% (n=6), respectively. CONCLUSIONS: The established method is simple, accurate and reproducible. It can be used for simultaneous determination of isoquercitrin, astragalin and salvianolic acid B in M. oleifera leaves granules.