Simultaneous Determination of 4 Triglyceride Anti-tumor Components in Coix lacryma Seed Oil by Quatitative Analysis of Multi-components by Single Marker
- VernacularTitle:一测多评法同时测定薏苡仁油中4种甘油三酯类抗肿瘤成分的含量
- Author:
Renjie WU
1
;
Pingcui XU
1
,
2
;
Dan SHOU
1
,
2
;
Gonghua LI
1
;
Qingshan LAN
3
;
Nani WANG
1
Author Information
1. TCM Research Center，Zhejiang Academy of Traditional Chinese Medicine，Hangzhou 310007，China
2. School of Pharmacy，Zhejiang University of TCM，Hangzhou 310053，China
3. Beijing Huamiao Pharmaceutical Ltd. Company，Beijing 102600，China
- Publication Type:Journal Article
- Keywords:
HPLC;
Quatitative analysis of multi-components by single marker;
Coix lacryma seed oil;
Triglyceride;
Relative correction factor
- From:
China Pharmacy
2019;30(10):1375-1380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for simultaneous determination of 4 triglyceride anti-tumor components in Coix lacryma seed oil. METHODS： HPLC-ELSD was adopted. The determination was performed on Inertsil ODS-3 C18 column with mobile phase consisted of acetonitrile-isopropanol （57 ∶ 43， V/V） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃， and the sample size was 10 μL. Evaporative light scattering detector was used， the drift tube temperature was 70 ℃， and the gas flow rate was 2 L/min. Using glycerol trioleateas internal standard， relative correction factors （RCF） of linolein trilinolein， 1，2-linoleic acid-3-palmitic acid glyceride and 1-palmitic acid-2-oleic acid-3-linoleic acid glyceride were calculated respectively. The contents of above 3 components in C. lacryma seed oil were calculated by RCF. The contents of 4 components in C. lacryma seed oil were determined by external standard. The results of content determination by quatitative analysis of multi-components by single marker （QAMS） were compared with external standard method. RESULTS： The linear ranges were 0.15-4.50 μg for linolein trilinolein， 0.15-4.50 μg for 1，2-linoleic acid-3-palmitate， 0.35-10.50 μg for 1-palmitic acid-2-oleic acid-3-linoleic acid glyceride， 0.35-10.50 μg for glycerol trioleate （r≥0.999 5）. The limits of quantification were 0.13， 0.06， 0.07， 0.12 μg. The limits of detection were 0.04， 0.02， 0.02， 0.03 μg， respectively. RSDs of precision， stability， and repeatability tests were less than 2.0％（n＝6）. The average recoveries were 95.43％-102.67％（RSD＜2.0％， n＝6）. Average RCFs of linolein trilinolein， 1，2- linoleic acid-3-palmitic acid glyceride and 1-palmitic acid-2- oleic acid-3-linoleic acid glyceride were 0.31， 0.88， and 1.21， respectively. RCFs reproducibility was perfect under different experiment conditions. There was no significant difference in results of content determination between QAMS and external standard method （P＞0.05）. CONCLUSIONS： The method is simple， rapid， accurate and reliable. It is used for simultaneous determination of linolein trilinolein， 1， 2-linoleic acid-3-palmitate， 1-palmitic acid-2-oleic acid-3-linoleic acid glyceride and glycerol trioleateas in C. lacryma seed oil.
