Effect of Le ’ermai Capsule on Protein Expression of HO- 1,SOD1,SOD2 and Nrf 2 in the Cerebral Cortex of Cerebral Ischemia/Reperfusion Injury Rats
- VernacularTitle:乐尔脉胶囊对脑缺血再灌注损伤大鼠大脑皮层组织中HO-1、SOD1、SOD2和Nrf2蛋白表达的影响
- Author:
Ningjuan CHI
1
;
Mingyi LIU
1
;
Juan FENG
1
;
Xiaopeng SHI
1
;
Zhifu YANG
1
;
Aidong WEN
1
Author Information
1. Dept. of Pharmacy,the First Affiliated Hospital of Air Force Military Medical University,Xi’an 710032,China
- Publication Type:Journal Article
- Keywords:
Le’ermai capsule;
Cerebral ischemia/reperfusion injury;
Oxidative stress;
HO-1;
SOD1;
SOD2;
Nrf2;
Rat
- From:
China Pharmacy
2019;30(11):1525-1529
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the effects and mechanism of Le’ermai capsule on oxidative stress injury in the cerebral cortex of cerebral ischemia-reperfusion injury rats. METHODS: Totally 65 rats were randomly divided into sham operation group (normal saline), model group (normal saline), positive control group (Naoxintong capsule, 3.40 g/kg), Le’ermai capsule high-dose and low-dose groups (0.37, 0.18 g/kg), with 13 rats in each group. Except that sham operation group received sham operation, and middle cerebral artery occlusion/reperfusion (MCAO/R) model was induced by suture-occluded method. 24 h after modeling, they were given relevant medicine intragastrically, once a day, for consecutive 7 d. 2 h after last administration, cerebral ischemic area was determined by TTC staining. The pathological changes of cerebral tissue were observed by HE staining. The protein expressions of HO-1, SOD1, SOD2 and Nrf2 in cerebral cortex were detected by Western blot assay. RESULTS: Compared with sham operation group, the area of cerebral ischemic was increased significantly in model group(P<0.05), interstitial edema was serious, inflammatory cell infiltration increased significantly; and the protein expression levels of HO-1 and SOD1 in cortex tissue were significantly increased (P<0.05), and the protein expression levels of SOD2 and Nrf2 were significantly decreased (P<0.05). Compared with model group, the area of cerebral ischemic area of Le’ermai capsule high-dose and low-dose groups were significantly decreased (P<0.05). The interstitial edema, inflammatory cell infiltration and microcytes proliferation were decreased. The protein expression levels of HO-1, SOD1, SOD2 and Nrf2 in the cerebral cortex tissue were significantly increased (P<0.05). CONCLUSIONS:Le’ermai capsule can improve cerebral ischemia/reperfusion injury to certain extent, and which may be associated with up-regulating the protein expression of antioxidant factors HO-1, SOD1, SOD2 and Nrf2 in the cortex.