Study on the Separation and Purification Technology of Total Flavonoids from Sparganium stoloni by Box-Behnken Design- response Surface Methodology
- VernacularTitle:Box-Behnken设计-响应面法优化三棱总黄酮的分离纯化工艺研究
- Author:
Yu KUANG
1
;
Yongyan ZHAO
1
;
Peiyu HE
1
;
Junyin ZHANG
1
;
Yan ZHOU
1
;
Teng PENG
1
;
Chenghao YU
2
Author Information
1. School of Pharmacy,Chengdu University of TCM/National Key Lab Breeding Base of TCM Resource System Study and Development Utilization/Key Lab of TCM Standardization,Ministry of Education,Chengdu 611137,China
2. College of Basic Medicine,Chengdu Uni versity of TCM,Chengdu 611137,China
- Publication Type:Journal Article
- Keywords:
Total flavonoids of Sparganium stoloniferum;
Purification technology;
Macroporous adsorption resin;
Box-Behnken design-response surface methodology
- From:
China Pharmacy
2019;30(11):1502-1506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize the purification technology of total flavonoids from Sparganium stoloniferum. METHODS: Separation and purification by macroporus adsorption resin, using sample solution pH, flow rate and concentration of eluent, the purification rate of total flavonoids as evalution indexes, the purification technology of total flavonoids from S. stoloniferum were optimized by Box-Behnken design-response surface methodology based on single factor test. Validation test was conducted. RESULTS: The optimal purification technology was sample solution pH 4.8, flow rate of eluent 2.0 BV/h, concentration of eluent 72%. The purification rate of total flavonoids in 3 batches of samples was 72.34% (RSD=1.77%, n=3) in validation test, relative errors of which to predicted value (73.99%) was 2.13%. CONCLUSIONS: The optimal purification technology is stable and feasible, and can be used for the purification of total flavonoids from S. stoloniferum.