Study on Optimization of the Extraction Technology of Total Vitamin E in Euryale ferox by Box-Behnken Design-response Surface Methodology
- VernacularTitle:Box-Behnken设计-响应面法优化芡实中总维生素E的提取工艺研究
- Author:
Hong WANG
1
,
2
;
Qinan WU
2
;
Yongwei CUI
1
;
Chengying WU
2
;
Bei SHEN
2
;
Xiuhe FAN
2
Author Information
1. Dept. of Pharmacy，Nanjing Lishui District Hospital of TCM，Nanjing 211200，China
2. College of Pharmacy，Nanjing University of TCM，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
Euryale ferox;
Vitamin E;
Optimization;
Extraction technology;
Box-Behnken design-response surface methodology
- From:
China Pharmacy
2019;30(11):1497-1501
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To optimize the extraction technology of total vitamin E in Euryale ferox. METHODS： With the extraction amount of total vitamin E as reference index， using extraction time， extraction times， ultrasound power and comminution degree as reference factors， single fator test and Box-Behnken design-response surface methodology was used to optimize the extraction technology of total vitamin E from E. ferox. The validation tests were conducted for 3 times （the amounts of E. ferox were 2.0， 20.0， 40.0 g）. RESULTS： The optimal extraction technology of vitamin E included that extraction time of 80 min， extraction times of 3 times， ultrasound power of 240 W， comminution degree of 80 mesh. In validation test， extraction rates of total vitamin E were 2.063， 2.103， 2.085 mg/g （RSD＝2.6％， 1.5％， 1.3％， n＝3）， the relative errors of which to predicted value （2.092 mg/g） were 0.14％， 0.53％ and 0.33％， respectively. CONCLUSIONS： The optimal extraction technology is reasonable， stable and feasible， and can be used for the extraction of total vitamin E in E. ferox.
