Simultaneous Determination of 6 Components in Chaihuang Tablets by HPLC-DAD
- VernacularTitle:HPLC-DAD法同时测定柴黄片中6种成分的含量
- Author:
Changling DING
1
;
Junfeng CUI
2
;
Xiaorong SHI
1
;
Yangyang XU
1
;
Wenna CHENG
1
;
Jinjie ZHANG
1
;
Li ZHAO
3
Author Information
1. Dept. of Pharmacy，the Affiliated Hospital of Binzhou Medical College，Shandong Binzhou 256603，China
2. Binzhou Center for Food and Drug Control，Shandong Binzhou 256618，China
3. Dept. of Pain，the Affiliated Hospital of Binzhou Medical College，Shandong Binzhou 256603，China
- Publication Type:Journal Article
- Keywords:
HPLC-DAD;
Chaihuang tablets;
Content determination;
Baicalin;
Wogonoside;
Baicalein;
Wogonin;
Saikosaponin a;
Saikosaponin d
- From:
China Pharmacy
2019;30(11):1477-1481
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for simultaneous determination of 6 components in Chaihuang tablets， such as baicalin， wogonoside， baicalein， wogonin， saikosaponin a and saikosaponin d in Chaihuang tablets. METHODS： HPLC-DAD method was used to detect 3 batches of Chaihuang tablets from same manufacturers. The determination was performed on Agilent Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-triethylamine phosphate aqueous solution （pH adjusted to 7.0， gradient elution） at flow rate of 1.0 mL/min. The detection wavelengths were set at 210 nm （saikosaponin a， saikosaponin d） and 277 nm （baicalin， wogonoside， baicalein， wogonin）. The column temperature was 30 ℃， and sample size was 5 μL. RESULTS： The linear ranges of baicalin， wogonoside， baicalein， wogonin， saikosaponin a and saikosaponin d were 0.379 5-7.590 4 μg， 0.082 96-1.659 2 μg， 0.039 39-0.787 8 μg， 0.040 72-0.814 4 μg， 0.040 45-0.809 0 μg， 0.038 63-0.772 6 μg （all r≥0.999 3）， respectively. The limits of detection were 0.008， 0.007， 0.005， 0.005， 0.020 and 0.018 μg/mL. The limits of quantitation were 0.025， 0.022， 0.015， 0.015， 0.060， 0.054 μg/mL. RSDs of precision， reproducibility and stability tests （48 h） were all lower than 1.5％ （n＝6）. Average recoveries were 98.46％， 97.06％， 100.90％， 96.13％， 96.91％， 96.57％ （RSD＜2.0％， n＝6）. CONCLUSIONS： Established method is simple， accurate and reproducible for 6 components in Chaihuang tablets， and can be used for quality control of the tablet.
