Study on the Optimization of Extraction Technology of Qiwei Chanshen Formula by Orthogonal Test Based on Information Entropy Theory
- VernacularTitle:基于信息熵赋权法的正交试验优化七味蟾参方提取工艺研究
- Author:
Zengzhi XING
1
,
2
;
Shuai LI
1
;
Aijun ZHANG
1
Author Information
1. Institute of Traditional Chinese Medicine，Sichuan Academy of Chinese Medicine Sciences，Chengdu 610041，China
2. School of Traditional Chinese Medicine，Guangdong Pharmaceutical University，Guangzhou 510006，China
- Publication Type:Journal Article
- Keywords:
Qiwei chanshen formula;
Information entropy theory;
Orthogonal test;
Polysaccharide;
Camptothecin;
Ginsenoside Rb1;
Fingerprint;
Similarity;
Extraction technology
- From:
China Pharmacy
2019;30(3):376-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To optimize the water extraction technology of Qiwei chanshen formula. METHODS： The content of polysaccharides from Gekko japonicus and Panax quinquefolium was determined by UV spectrophotometry， the contents of camptothecin from Camptotheca acuminata and ginsenoside Rb1 from P. quinquefolium were determined by HPLC. The extraction rate of above three components， dry extractum yield and HPLC fingerprint similarity were used as evaluation indicators； information entropy theory was used to determine the weight of each indicator so as to calculate comprehensive score. L9（34） orthogonal test was used to screen the optimal extraction technology of Qiwei chanshen formula with decoction time， water volume and decoction times as factors. Validation test was also performed. RESULTS： The optimal extraction technology included 10-fold water， decocting for 3 times， 1.0 h each time. The results of validation test showed that the average extraction rate of polysaccharide， camptothecin and ginsenoside Rb1 were 74.306％， 13.860％ and 52.958％， respectively. The average dry extractum yield was 16.150％， the average value of fingerprint similarity was 0.991 （all RSDs＜3.0％， n＝3）. CONCLUSIONS： The optimized extraction technology is reproducible， stable and feasible， providing reference for the subsequent development and industrial production of the formula.
