Optimization of Extraction Technology of Osthole from Pine Needles of Cedrus deodara by Box-Behnken Design-response Surface Methodology
- VernacularTitle:Box-Behnken设计-响应面法优选雪松松针中蛇床子素的提取工艺
- Author:
Bin FAN
1
;
Lin LI
2
;
Xiaofeng SHI
1
,
2
;
Wei SHEN
1
;
Quhuan MA
1
;
Dongyan LIU
1
;
Xindi WANG
1
Author Information
1. Gansu Provincial Academy of Medical Science，Lanzhou 730050，China
2. School of Pharmacy，Gansu University of TCM，Lanzhou 730030，China
- Publication Type:Journal Article
- Keywords:
Pine needles of Cedrus deodara;
Osthole;
Extraction technology;
Box-Behnken design;
Response surface methodology
- From:
China Pharmacy
2019;30(3):359-363
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To optimize the extraction technology of osthole from the pine needles of Cedrus deodara. METHODS： HPLC method was adopted to determine the content of osthole. Based on single factor test， ethanol volume fraction， extraction time and material-solvent ratio were selected as influential factors， and the content of osthole was selected as response value. Box-Behnken design-response surface methodology was used to optimize the extraction technology of osthole in pine needles of C. deodara. Validation test was conducted. RESULTS： The optimal extraction technology was as follows as ethanol volume fraction of 88％， material-solvent ratio of 1 ∶ 20 （g/mL）， extracting for 2 times， lasting for 57 min each time. Under this technology， average content of osthole was 0.675 7 mg/g （RSD＝1.78％， n＝3）， and the relative error of which to predicted value 0.680 9 mg/g was 0.59％. CONCLUSIONS： The optimal extraction technology is simple and feasible，and it can be used for the extraction of osthole from the pine needles of C. deodara.
