Hamilton-Thorne Integrated Visual Optical System Ⅰ (IVOSⅠ) versus IVOS Ⅱ for human sperm analysis.
- Author:
Hua ZHOU
1
;
Qing LI
1
;
Li-Yuan GUO
1
;
Jing-Ru YUAN
1
;
Min LIU
1
;
Xiao-Tian ZHAO
2
;
Guo-Dong YE
2
Author Information
1. Research Institute of Obstetrics and Gynecology / Key Lab of Reproduction and Genetics of Guangdong Higher Education Institutions, The Third Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, China.
2. School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 511436, China.
- Publication Type:Journal Article
- Keywords:
comparative analysis;
quality control;
computer-assisted sperm analysis
- From:National Journal of Andrology
2019;25(5):315-321
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the computer-assisted sperm analysis (CASA) systems Hamilton-Thorne Integrated Visual Optical System Ⅰ (IVOSⅠ) and IVOS Ⅱ after verifying the performance of the latter so as to ensure the accuracy of the results of analysis.
METHODS:Based on the criteria established in the 5th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen (WHO 5th Ed), we compared the main semen parameters obtained from IVOSⅠ with those generated by IVOS Ⅱ, and examined the consistency between the results of the two sperm analyzers.
RESULTS:The linear relationship of the outlier test, bias estimation and scatter plot and the results of the outlier test of the two systems all met the requirements of comparison analysis and showed an obvious correlativity. The application scope of the results obtained from the apparatus indicated a reasonable value range, with r = 0.988 for sperm concentration, r = 0.975 for sperm progressive motility (PR), and r = 0.981 for total sperm motility. Evaluation of the acceptability of the predicted bias showed that the allowable total error (TEa) to be 6.67% with sperm concentration at 12 × 106 /ml and 2.34% with PR < 31%, their upper limit of the allowable error < 1/2. The results of IVOS Ⅱ conformed to the requirements of the WHO 5th Ed.
CONCLUSIONS:The main parameters derived from IVOSⅠ and IVOS Ⅱ are comparable and consistent, indicating that both can be used for the examination of semen samples.
