Expression and clinical significance of melanoma antigen A gene family in peripheral blood of esophageal carcinoma patients
10.3872/j.issn.1007-385x.2018.08.009
- VernacularTitle:黑色素瘤相关抗原A基因家族在食管癌患者外周血中的表达及其临床 意义
- Author:
GU Lina
1
;
YIN Danjing
1
;
SANG Meixiang
2
,
3
;
LIU Fei
1
;
WU Yunyan
1
;
LIU Shina
4
;
LI Juan
1
;
SHAN Baoen
2
,
3
Author Information
1. a. Scintific of Research Center, Tumor Research Institute, Fourth Hospital of Hebei Medical University
2. (a. Scintific of Research Center
3. b. Department of Immunology, Tumor Research Institute, Fourth Hospital of Hebei Medical University
4. b. Department of Immunology, Tumor Research Institute, Fourth Hospital of Hebei Medical University,
- Publication Type:Journal Article
- Keywords:
esophageal carcinoma;
melanoma antigenA;
peripheral blood;
prognosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(8):803-809
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the expression of melanoma antigen A family（MAGE-As）in the peripheral blood of patients with esophageal carcinoma (EC), and to analyze its correlations to the clinicopathological features and the prognosis of EC patients. Methods: mRNA expression of MAGE-As in peripheral blood from 153 EC patients and 30 healthy donors was detected using multiplex semi-nested PCR. In addition, restriction endonuclease treatment was used to determine the expression of MAGE-As family members, including MAGE-A1, A2, A3, A4 and A6. Results: The positive expression of MAGE-As was observed in 30 of 153 EC patients (19.61%) in peripheral blood. The positive expression rate of MAGE-A1, A2, A3, A4, A6 was 10.46% (16/153), 16.34%(25/153), 9.8% (15/153), 11.11% (17/153) and 18.30%(28/153), respectively. Additionally, the expression of MAGE-As was positively associated with clinical stage, lymphatic metastasis and distant metastasis (all P<0.05). The positive expressions of MAGE-As and its sub-type genes were all associated with low 5-year overall survival of ES patients (all P<0.05). Expression of MAGE-As, tumor volume, lymphatic metastasis and distant metastasis can be used as independent prognostic factors for the survival of EC patients (all P<0.01). Conclusion: The expression of MAGE-As in peripheral blood of EC patients was associated with the prognosis of EC, and may be used as an important indicator for the prognosis of esophageal carcinoma.
- Full text:20180809.pdf
