Over-expression of miR-204 suppresses proliferation and invasion of retinoblastoma cells and its possible mechanism
10.3872/j.issn.1007-385x.2018.08.007
- VernacularTitle:miR-204过表达抑制成视网膜细胞瘤细胞的增殖与侵袭及其可能的机制
- Author:
WANG Jianmin
1
,
2
;
ZHANG Jiabin
1
,
2
;
LIU Jiehui
3
,
4
;
GAO Peng
1
,
2
Author Information
1. Department of Ophthalmology, Laiwu People'
2. s Hospital
3. Department of Ophthalmology, Ji'
4. nan Central Hospital Affiliated to Shandong University
- Publication Type:Journal Article
- Keywords:
retinoblastoma；miR-204;
proliferation;
migration；invasion;
high mobility group AT-hook 2 gene (HMGA2)
- From:
Chinese Journal of Cancer Biotherapy
2018;25(8):791-796
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of miR-204 on the proliferation and invasion of retinoblastoma (RB) cells and to explore the potential regulatory mechanism. Methods: The expression level of miR-204 in RB cell lines (Y79, SO-RB50, and HXO-Rb44) as well as in normal human retinal pigment epithelial cell line hTERT RPE-1 was detected using qRT-PCR. The Y79 cells were divided into two groups (negative control group and miR-204 group) by respectively transfecting Y79 cells with NC-mimics and miR-204 mimics using liposome transfection method. The effects of miR-204 on Y79 cell proliferation was detected with CCK-8 assay; while the effect of miR-204 on migration and invasion of Y79cellsweredeterminedbycellscratchassayandTranswellassay,respectively.Besides, thepotentialtargetgeneofmiR-204waspredictedbybioinformatics;and the influence of miR-204 on the expression of high mobility group AT-hook 2 gene (HMGA2) at both mRNA and protein levels was detected using qRT-PCR and Western blotting, respectively. Results: miR-204 expression in RB cell lines Y79, SO-RB50 and HXO-Rb44 was remarkably lower than that in normal human retinal pigment epithelial cell line hTERT RPE-1 (P<0.01). miR-204 expression in Y79 cells was markedly up-regulated after transfection with miR-204 mimics (P<0.01) along with significantly reduced cell proliferation, migration and invasion capacities (all P<0.01), and mRNA and protein expressions of HMGA2 were also outstandingly reduced (P<0.01). Conclusion: miR-204 is lowly expressed in RB cell lines; in addition, miR-204 over-expression can suppress RB cell proliferation, migration and invasion, the mechanism of which might be related to down-regulation of the expression of HMGA2.
- Full text:20180807.pdf
