miR-124 regulates autophagy to inhibit invasion and migration of esophageal cancer KYSE170 cells by targeting BECN1
10.3872/j.issn.1007-385x.2018.08.005
- VernacularTitle:miR-124通过靶向BECN1基因调控细胞自噬抑制食管癌KYSE170细胞 的侵袭和迁移
- Author:
LIU Fei
1
;
LIU Sihua
1
;
LIU Shina
1
;
GU Lina
1
;
MENG Lingjiao
1
;
YIN Danjing
1
;
ZHANG Jiandong
1
;
WU Yunyan
1
;
SANG Meixiang
1
Author Information
1. Scientific Research Center, the Fourth Hospital of Hebei Medical University
- Publication Type:Journal Article
- Keywords:
miR-124;
BECN1;
esophageal carcinoma;
KYSE170 cell;
autophagy;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2018;25(8):778-784
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of miR-124 on the invasion and migration of esophageal cancer KYSE170 cells by regulating autophagy. Methods: miR-124 mimic was transfected into esophageal cancer KYSE170 cells. Transwell assay was used to detect the change of invasion and migration ability of cells. Dual luciferase reporter gene assay was used to verify the targeted regulation of BECN1 (Beclin1) by miR-124, and Western blotting was used to analyze the expressions of BECN1, P62 and LC3 protein. siRNA targeting BECN1 was transfeted into KYSE170 cells, and then the cell invasion and migration ability was calculated by Transwell assay. The expressions of BECN1, P62 and LC3 protein were detected by Western blotting. miR-124 mimic and BECN1 over-expression plasmid were co-transfected into KYSE170 cells, and then Transwell assay was used to detect the changes of cell invasion and migration ability, and Western blotting to examine the expression levels of autophagy-related gene. Results: The invasion and migration ability of KYSE170 cells were significantly inhibited after transfection with miR-124 mimic (All P<0.05). The expression of autophagyrelated protein P62 was increased, and the expression of BECN1 and LC3 was significantly decreased (All P<0.01); in addition, the activity of luciferase reporter gene was also significantly reduced (P<0.01). Silencing BECN1 expression inhibited the invasion and migration of esophageal cancer KYSE170 cells (P<0.01). However, after co-transfection with BECN1 over-expression plasmids, the effects of miR-124 mimic on the autophagy, invasion and migration of esophageal carcinoma KYSE170 cells were significantly weakened (P<0.01), it was also accompanied with lower P62 expression, and higher LC3 expression (P<0.01). Conclusion: miR-124 mimic can inhibit the invasion and migration of esophageal carcinoma cells. The mechanism may be related to the autophagy-related gene BECN1 expression.
- Full text:20180805.pdf
