Effect of myeloid-derived suppressor cells on B cell function in mice bearing breast cancer
10.3872/j.issn.1007-385x.2018.08.004
- VernacularTitle:荷乳腺癌小鼠髓源抑制性细胞对B细胞功能的影响
- Author:
LIU Min
1
;
WANG Jian
1
;
SUN Qian
1
;
YU Wenwen
1
;
WEI Feng
1
;
REN Xiubao
1
,
2
Author Information
1. a. Biotechnology Lab, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin Clinical Research Center for Malignant Cancer, Key Laboratory of Cancer Immunology and Biotherapy of Tianjin City
2. b. Department of Biotherapy, Cancer Hospital of Tianjin Medical University
- Publication Type:Journal Article
- Keywords:
myeloid-derived suppressor cell;
B cell;
breast cancer;
tumor immunity
- From:
Chinese Journal of Cancer Biotherapy
2018;25(8):772-777
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of myeloid-derived suppressor cells (MDSCs) from mice bearing breast cancer on the function of normal B cells. Methods:ABABL/c mouse 4T1 breast cancer model was established. The spleen MDSCs of tumor-bearing mouse and normal mouse spleen B cells were sorted by magnetic beads, and the sorted MDSCs and B cells were co-incubated. Flow cytometry was used to test the effect of MDSCs on the expressions of B cell surface molecules, including PD-1, PD-L1, CTLA-4, CCR6, CD62L and MHCⅡ; ELISA assay was used to detect the secretion of IgA, IgM and IgG by B cells; BrdU kit was used to detect B cell proliferation; andAnnexin V/PI staining was used to detect B cell apoptosis. B cells in the co-culture system were again sorted by magnetic beads and were then co-cultured with T cells; BrdU kit was used to detect T cell proliferation, and Annexin V/PI was used to detect T cell apoptosis. Results: Compared with B cell control group, the expression of PD-L1 on B cells in B+MDSC group was increased (P<0.01), while the expressions of PD-1, CTLA-4, CCR6, CD62L and MHC Ⅱ were all decreased (all P<0.01); The IgA, IgM and IgG secreted by B cells were significantly increased (all P<0.01); the proliferation of B cells was increased (P<0.01) and the apoptosis was decreased (P<0.01). Compared with the T cell control group, the proliferation of T cells in the B+MDSC (1:5) +T group was significantly reduced (P<0.01); however, there was no significant difference in T cell apoptosis. Conclusion: MDSCs from breast cancer bearing mice promotes B cell proliferation and inhibits B cell apoptosis, and the MDSC-induced B cells can inhibit T cell proliferation.
- Full text:20180804.pdf
