Study on Protective Effects of Polygonum orientale Extract on Myocardial Ischemia-reperfusion Injury Model Rats
- VernacularTitle:荭草花提取物对心肌缺血再灌注损伤模型大鼠的保护作用研究
- Author:
Junhong LIU
1
;
Changquan WANG
2
,
3
;
Tao XIA
2
,
3
;
Wenying XIANG
2
,
3
;
Lin ZHENG
2
;
Yongjun LI
4
;
Yueting LI
2
Author Information
1. Guizhou Province Center for Food and Drug Evaluation and Inspection，Guiyang 550004，China
2. Guizhou Provincial Key Lab of Pharmaceutics/State Key Lab of Functions and Applications of Medicinal Plants，Guizhou Medical University，Guiyang 550004，China
3. School of Pharmacy，Guizhou Medical University，Guiyang 550004，China
4. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM（Ministry of Education ），Guizhou Medical University，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Polygonum orientale extract;
Myocardial ischemia and reperfusion injury;
Oxidative damage;
Rats
- From:
China Pharmacy
2019;30(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study the protective effects of Polygonum orientale extract on myocardial ischemia-reperfusion injury （MIRI） model rats， and to provide reference for it’s deeply development of medicinal source. METHODS： Totally 24 rats were randomly divided into sham operation group （normal saline）， model group （normal saline）， Compound danshen tablet group （positive group， 0.17 g/kg） and P. orientale extract group （86 g/kg， calculated by crude drug）， with 6 rats in each group. All groups were given drugs 2 mL/100 g intragastrically once a day. After 4 d of consecutive administration， MIRI model was induced by the left anterior descending branch of arteria coronaria in all groups except for sham operation group. 24 h after reperfusion， they were given related medicine again. After medication， the changes of electrocardiogram ST segment were monitored in each group. The plasma levels of LDH， CK-MB， CK， cTn-I， SOD and NO were detected in each group. The myocardial infarction rate in each group was calculated and the pathomorphological changes in the myocardium were observed. RESULTS： Compared with sham operation group， ST segment of myocardial electrocardiogram was increased in model group （P＜0.01）. The plasma levels of LDH， CK， CK-MB and cTn-I were increased significantly （P＜0.01）， while the plasma levels of SOD and NO were decreased significantly （P＜0.01）. The rate of myocardial infarction was increased significantly （P＜0.01）， and pathomorphological changes were observed in myocardial tissue such as infiltration of inflammatory cells and loose cytoplasm of cardiac myocytes. Compared with model group， ST segment of myocardial electrocardiogram was decreased significantly in Compound danshen tablet group and P. orientale extract group （P＜0.05）； the plasma levels of LDH， CK， CK-MB and cTn-I were decreased significantly （P＜0.05）， while the plasma levels of SOD and NO were increased significantly （P＜0.05）； the rate of myocardial infarction was decreased significantly （P＜0.05）， and inflammatory cell infiltration and tissue edema in myocardium were relieved to varying degrees. CONCLUSIONS： The protective effect of P. orientale extract protect on MIRI may be exerted by anti-oxidative damage.
