Simultaneous Determination of 6 Kinds of Constituent in Dahuang Zhechong Pills by HPLC-PDA
- VernacularTitle:HPLC-PDA法同时测定大黄蛰虫丸中6种成分的含量
- Author:
Guiping ZHANG
1
;
Dongxu WANG
2
Author Information
1. Nanyang Institute for Food and Drug Control，Henan Nanyang 473061，China
2. Dept. of Laboratory，Nanyang Second General Hospital，Henan Nanyang 473000，China
- Publication Type:Journal Article
- Keywords:
Dahuang zhechong pills;
Amygdalin;
Paeonifiorin;
Baicalin;
Glycyrrhizic acid;
Emodin;
Chrysophanol;
HPLC;
Content determination
- From:
China Pharmacy
2019;30(1):54-58
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for amygdalin， paeoniforin， baicalin， glycyrrhizic acid， emodin and chrysophanol in Dahuang zhechong pills. METHODS： HPLC method was adopted. The determination was performed on Welchrom C18 with mobile phase consisted of methanol-0.1％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃， and the detection wavelengths were set at 210 nm （amygdalin）， 230 nm （paeoniforin， baicalin） and 250 nm [glycyrrhizic acid （calculated with ammonium glycyrrhizinate）， emodin， chrysophanol]. The sample size was 10 μL. RESULTS： The linear ranges of amygdalin，paeonifiorin，baicalin，ammonium glycyrrhizinate， emodin and chrysophanol fell within the ranges of 21.028-157.71，2.052-90.390， 34.288-257.16， 8.252 0-61.890， 3.272 0-24.540，4.768 0-35.760 μg/mL （all r≥0.999 2）. The limits of detection were 0.105 0， 0.121 0， 0.068 6， 0.082 5， 0.024 6， 0.017 9 μg/mL. The limits of quantitation were 0.263 0， 0.362 0， 0.171 0， 0.268 0， 0.065 5， 0.047 7 μg/mL. RSDs of precision， stability（12 h）and reproducibility tests were lower than 2.00％ （n＝6）. The average recoveries were 96.01％-100.76％ （RSD＝1.63％，n＝6）， 97.09％-101.86％ （RSD＝1.50％，n＝6）， 99.70％-101.99％（RSD＝0.82％，n＝6），96.29％-99.52％（RSD＝1.35％，n＝6）， 98.47％-102.14％ （RSD＝1.35％， n＝6） and 97.19％-99.16％（RSD＝0.69％，n＝6），respectively. CONCLUSIONS： The method is accurate， simple and reproducible for simultaneous determination of amygdalin， paeoniforin， baicalin， glycyrrhizic acid， emodin and chrysophanol in Dahuang zhechong pills.
