Simultaneous Determination of Ephedrine Hydrochloride ，Pseudoephedrine Hydrochloride ，Methamphetamine Hydrochloride and Paeoniflorin in Xiaoqinglong Granule by Micellar Capillary Electrophoresis Method
- VernacularTitle:胶束毛细管电泳法同时测定小青龙颗粒中的盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱与芍药苷含量
- Author:
Bo WANG
1
;
Xiang MENG
2
;
Xiaolong MENG
1
;
Ning XU
1
;
Yunfeng LI
1
;
Fanyun MENG
1
Author Information
1. Beijing Key Lab of TCM Protection and Utilization，Faculty of Geographical Science，Beijing Normal University，Beijing 100875，China
2. Institute of Clinical Basic Medicine for Chinese Medicine，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Journal Article
- Keywords:
Xiaoqinglong granule;
Micellar capillary electrophoresis;
Ephedrine hydrochloride;
Pseudoephedrine hydrochloride;
Methamphetamine hydrochloride;
Paeoniflorin;
Content determination
- From:
China Pharmacy
2019;30(1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish the method for simultaneous determination of ephedrine hydrochloride， pseudoephedrine hydrochloride， methamphetamine hydrochloride and paeoniflorin in Xiaoqinglong granule. METHODS： Micellar capillary electrophoresis （MCE） method was adopted. The optimum conditions for the separation were as follows as a fused silica capillary column as the separation channel， the buffer solution composed of 10 mmol/L borax-10 mmol/L SDS （95 ∶ 5， pH 10.5）， detection wavelength of 195 nm， separation voltage of 20 kV， capillary column temperature of 15 ℃，the sampling at a pressure for 0.5 psi×5 s. Two batches of Xiaoqinglong granules were collected from 2 manufacturers to determine the contents of ephedrine hydrochloride， pseudoephedrine hydrochloride， methamphetamine hydrochloride and paeoniflorin. The results of content determination were compared with the results determined by HPLC method stated in Chinese Pharmacopeia of 2015 edition. RESULTS： The linear range of ephedrine hydrochloride， pseudoephedrine hydrochloride， methamphetamine hydrochloride and paeoniflorin were 10-160， 10-160， 1-100， 10-500 μg/mL （r＝0.997 9-0.999 8）， respectively. RSDs of precision， reproducibility and stability tests were all ≤2.74％ （n＝5-6）. The average recoveries were 101.55％， 101.62％， 100.15％， 101.85％ （RSD≤3.94％， n＝6）， respectively. The contents of 4 components determined by micellar capillary electrophoresis were in accordance with the results of HPLC method. CONCLUSIONS： The established MCE method is simple， quick and sensitive， and can be used for simultaneous determination of 4 components mentioned above in Xiaoqinglong granule.
