Study on HPLC Fingerprint of Miao Medicine Hedyotis uncinella from Guizhou
- VernacularTitle:黔产苗药对坐叶药材HPLC指纹图谱研究
- Author:
Hua FENG
1
;
Liang LIU
2
;
Xiangpei WANG
3
;
Shunmei LIU
4
Author Information
1. TCM Section，Zunyi Institute for Food and Drug Control，Guizhou Zunyi 563002，China
2. Dept. of Pharmacy，Zunyi Medical and Pharmaceutical College，Guizhou Zunyi 563000，China
3. School of Pharmacy，Guiyang College of Traditional Chinese Medicine，Guiyang 550002，China
4. Pharmacy of Zunyi First People’s Hospital，Guizhou Zunyi 563002，China
- Publication Type:Journal Article
- Keywords:
Miao medicine from Guizhou;
Hedyotis uncinella;
HPLC;
Fingerprint
- From:
China Pharmacy
2019;30(2):232-235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish HPLC fingerprint of Miao medicine Hedyotis uncinella from Guizhou. METHODS： HPLC method was adopted. The determination was performed on Agilent Eclipse XDB C18 column with mobile phase consisted of acetonitrile-0.2％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 235 nm， and column temperature was 25 ℃. The sample size was 15 μL. Using rutin as reference， HPLC chromatograms of 10 batches of H. uncinella from different areas of Guizhou province were determined. Similarity Evaluation System for TCM Chromatographic Fingerprints （2004A edition） was used to identify common peaks and evaluate similarity. RESULTS： There were 12 common peaks in HPLC fingerprints of 10 batches of H. uncinella， and the similarity was higher than 0.90. After validation， HPLC chromatograms of 10 batches of sample were in agreement with control fingerprints. CONCLUSIONS： Established HPLC fingerprints can provide reference for the identification and quality evaluation of H. uncinella.
