Rapid Determination of Total Phenylethanoid Glycosides and Total Iridoid Glycosides in Rehmannia glutinosa by Near Infrared Spectroscopy
- VernacularTitle:近红外光谱法快速测定怀地黄中总苯乙醇苷、总环烯醚萜苷的含量
- Author:
Xiaotong GENG
1
;
Fengqing WANG
2
;
Xiuhong SU
1
;
Xiaosai TAO
1
;
Yajing LI
1
;
Jingwei LEI
1
;
Caixia XIE
1
Author Information
1. School of Pharmacy，Henan University of TCM，Zhengzhou 450046，China
2. College of Agriculture，Henan Agricultural University，Zhengzhou 450000，China
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa;
NIRS;
PLS;
Total phenylethanoid glycosides;
Total iridoid glycosides;
Rapid determination
- From:
China Pharmacy
2019;30(2):225-231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for rapid determination of total phenylethanoid glycosides and total iridoid glycosides in the root of Rehmannia glutinosa. METHODS： The contents of total phenylethanoid glycosides and total iridoid glycosides in medicinal material samples were determined by UV spectrophotometry. Quantitative model of total phenylethanoid glycosides and total iridoid glycosides in medicinal samples was established by NIRS-PLS method. The optimal pretreatment spectra were multivariate scattering correction combined with first derivative method， standard normalization combined with first derivative method. The optimum spectral ranged from 6 703.35-11 065.54 cm－1 and 3 999.63-9 102.36 cm－1. The optimum principal factor number were 10 and 7. RESULTS： The content determination of total phenylethanoid glycosides and total iridoid glycosides in medicinal material samples was proved to meet the requirements by methodological experience. The internal cross validation determination coefficients of total phenylethanoid glycosides and total iridoid glycosides were 0.998 2 and 0.980 9. The correction of root mean square error was 0.032 7 and 0.186 0. The root mean square error of prediction were 0.035 5 and 0.035 1. The root mean square error of cross validation were 0.256 9 and 0.574 3. The predicted values of total phenylethanol glycosides and total iridoid glycosides were 0.268％-1.636％ and 3.424％-6.978％， respectively； the determination value of them were 0.299％-1.629％ and 3.431％-6.952％， respectively； the absolute deviations were －0.042％-0.067％ and －0.111％-0.088％， respectively；the relative deviations were －0.819％-0.076％、－2.257％-1.672％， respectively；There was no statistical significance between predicted values and measured values （P＞0.05）. CONCLUSIONS： The method is accurate and simple. The method can be used for the rapid determination of total phenylethanoid glycosides and total iridoid glycosides in different germplasms of R. glutinosa.
