Establishment of HPLC Fingerprint and Principal Component Analysis of Xiaojin Capsules
- VernacularTitle:小金胶囊的HPLC指纹图谱建立及主成分分析
- Author:
Kaili MING
1
;
Yang XIANG
1
;
Yanxia YANG
1
;
Tian ZENG
1
;
Yatou WANG
1
;
Wei CAI
1
;
Wen ZHU
1
Author Information
1. Jianmin Pharmaceutical Gro up Co.，Ltd.，Wuhan 430052，China
- Publication Type:Journal Article
- Keywords:
Xiaojin capsules;
HPLC;
Fingerprint;
Principal component analysis
- From:
China Pharmacy
2019;30(2):188-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish HPLC fingerprint of Xiaojin capsules， and to conduct principal component analysis. METHODS： HPLC method was adopted. The determination was performed on Agilent TC-C18（2） column with mobile phase consisted of acetonitrile-0.2％ formic acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was 240 nm， and column temperature was 25 ℃. The sample size was 20 μL. Acetyl-11-keto-β-boswellic acid was used as reference and HPLC fingerprints of 15 batches of Xiaojin capsules were determined. The similarity evaluation of common peaks was conducted by using the TCM Chromatographic Fingerprint Similarity Evaluation System （2004A edition） to confirm common peaks. Principal component analysis was conducted by using Minitab 17.0 software. RESULTS： The similarity of 1 batch of sample was lower than 0.800； there was no common peaks No. 13， 14， 15 in HPLC chromatogram of the batch， compared with other 14 batches. There were 15 common peaks in the HPLC fingerprints of 14 batches of samples and three chromatographic peaks were identified， such as quercetin，amentoflavone， acetyl-11-keto-β-boswellic acid. The similarity of 14 batches ranged 0.889-0.990. Through principal component analysis， accumulative contribution rate of 2 principal component factors was 94.4％. It was indicated that the content change of corresponding components of common peaks No. 8， 9， 11， 12， 14 in samples was an important reason for the quality difference of samples， especially common peaks No.8， 9. CONCLUSIONS： The established HPLC fingerprint and principal component analysis can provide reference for quality evaluation of Xiaojin capsules.
