Improvement Effects of Bee Venom Plastics on Experimental Cerebral Thrombosis in Rats
- VernacularTitle:蜜蜂蜂毒涂膜剂经皮给药对实验性脑血栓大鼠的改善作用
- Author:
Miao HE
1
,
2
,
3
,
4
;
Zhixue ZHANG
1
,
2
,
4
;
Hairong ZHAO
1
,
2
,
3
,
4
;
Xiumei WU
1
,
2
,
3
,
4
;
Yu ZHAO
1
,
2
,
3
,
4
;
Chenggui ZHANG
1
,
2
,
3
Author Information
1. Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D，Dali University，Yunan Dali 671000，China
2. Institute of Insect Biology and Medicine，Dali University，Yunnan Dali 671000，China
3. Southwest 2011 Collaborative Innovation Center for Development and Utilization of Medicinal Entomoceutics and Arachnoidea Resource，Dali University，Yunan Dali 671000，China
4. National-local Joint Engineering Research Center of Medicinal Specialty Entomoceutics，Dali University，Yunnan Dali 671000，China
- Publication Type:Journal Article
- Keywords:
Bee vonom;
Plastics;
Cerebral thrombosis;
Cerabral edema;
Permeability;
Hemorheology;
Coagulation function
- From:
China Pharmacy
2019;30(2):182-187
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study the improvement effects of Bee venom（BV） plastics on experimental cerebral thrombosis in rats. METHODS： Totally 96 SD rats were randomly divided into sham operation group （normal saline）， model group （plastics blank matrix）， Nimodipine group （positive drug， 4.00 mg/kg） and BV plastics low-dose， medium-dose， high-dose groups （1.67， 3.33， 6.67 mg/kg）， with 16 rats in each group. Rats in sham operation group and Nimodipine group were given medicine intragastrically， while rats in model group and BV plastics groups were given medicine by transdermal smearing. After 5 days of continuous administration， the experimental cerebral thrombosis model was established by ligating the right external carotid artery and pterygomandibular artery， and injecting compound thrombus inducer into the internal carotid artery. The wet mass ratio of right brain to left brain was measured to investigate the degree of brain edema on the infarcted side. The content of Evans blue （EB） in the left and right hemispheres of rats was determined by ultraviolet spectrophotometry to investigate the cerebral vascular permeability. Blood rheology and coagulation function indicators of rats were measured. The pathological changes of brain tissue in rats were observed by HE staining， and the number of survival neuron cells was counted. RESULTS： Compared with the indexes of sham operation group， the cerebral thrombosis model was established successfully. Compared with model group， the area of blue staining in the right brain （infarcted side） of rats in BV plastics groups was significantly reduced， and the right brain/left brain wet mass ratio and the content of EB in the right brain tissue were significantly reduced （P＜0.05 or P＜0.01）. The whole blood viscosity and Casson viscosity of rats in BV plastics groups， and the plasma viscosity of rats in BV plastics medium-dose and high-dose groups decreased significantly （P＜0.01）. PT and APTT of rats were prolonged significantly in BV plastics medium-dose group （P＜0.01）. The pathological changes of brain tissue in rats in BV plastics groups were significantly alleviated. The arrangement of neuron cells was more orderly， the shape and structure of cells were clear， the nucleolus was clear， the membrane was intact， and the number of survival neuron cells was significantly increased （P＜0.01）. CONCLUSIONS： BV plastics can alleviate brain edema， inhibit cerebral vascular permeability， improve hemorheology and coagulation function indicators of rats after the formation of cerebral thrombosis， and alleviate nerve cell injury after ischemia.
