Expression and Purification of Recombinant Mayaro Virus Envelope Glycoproteins E1 and E2 to Develop a Mayaro Virus Detection System
10.4167/jbv.2020.50.1.025
- Author:
So Yeon YI
1
;
Kyungah YOON
;
Jungsun KWON
;
Kyoon Eon KIM
;
Kyoungsook PARK
;
Yong Beom SHIN
Author Information
1. BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. marsp@kribb.re.kr, ybshin@kribb.re.kr
- Publication Type:Original Article
- From:Journal of Bacteriology and Virology
2020;50(1):25-34
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mayaro virus (MAYV) is a mosquito-transmitted alphavirus that produces an acute, usually non-fatal, febrile illness including Mayaro fever. Like other alphaviruses, the MAYV E1 and E2 envelope glycoproteins are major viral surface antigens that play a key role in host recognition and infection. Here, we report expression and purification methods for recombinant MAYV E1 (rE1) and rE2 using a baculovirus system. Enzyme-linked immunosorbent assays (ELISA) revealed that rE1 and rE2 were antigenic and reacted with human anti-MAYV IgG and IgM. Cross-reactivity was also confirmed with human anti-Chikungunya virus (CHIKV) IgG and IgM. Furthermore, we developed an immunochromatographic strip test (IST) with rE2 to diagnose MAYV infection. Thus, purified rE2 may be valuable tool for rapidly diagnosing MAYV infection.
