Morphological and Quantitative Analysis on the Expression of Bax in the Rat Prostate following Castration.
- Author:
Moon Soo PARK
1
;
Soo Woong KIM
;
Gheeyoung CHOE
;
Ahnkie LEE
;
Eunsik LEE
;
Si Whang KIM
Author Information
1. Department of Urology, Seoul Municipal Boramae Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Bax;
Prostate;
Apoptosis;
Castration;
Rat
- MeSH:
Adult;
Animals;
Apoptosis;
Blotting, Western;
Castration*;
Cell Death;
Humans;
Immunoglobulin G;
Male;
Prostate*;
Rats*
- From:Korean Journal of Urology
1998;39(7):622-626
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Bax promotes apoptosis and is up-regulated by p53, which is not required for androgen ablation-induced apoptosis of the prostatic cells. We investigated the change of lax during the involution of the rat prostate following castration. MATERIALS AND METHOD: Adult male rats were castrated and the ventral prostatic glands were removed daily for a week following castration. Western blot and immunohistochemical analysis of lax protein were performed using anti-bax rabbit polyclonal IgG. RESULTS: The prostatic weight decreased and the apoptotic cells began to appear 2 days following castration. Western blot analysis showed that lax was absent in the control prostate but began to increase 4 days following castration. Immunohistochemical analysis showed that bars was confined to the cells which had morphological characteristics of the late stage of programmed cell death. CONCLUSIONS: Present results suggest that bars might be involved in the late stage of castration-induced apoptosis of the rat prostatic cells.
